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Exome Assembly

DNASTAR's Lasergene Genomics Suite software allows users to effortlessly assemble exome data against a genomic reference sequence and perform comprehensive post-assembly analysis, including identifying SNPs and structural variants, and reviewing coverage. Features of the Lasergene Genomics Suite software include:

  • Support for both single-end and paired-end data
  • The ability to assemble multiplexed data or multiple samples simultaneously
  • Large-scale SNP comparisons across individuals and groups
  • Advanced gene filtering based on the level of disruption to each gene caused by variations
  • Support for all major sequencing technologies
    • For more information on using Lasergene Genomics Suite for your exome assembly projects, this brief video gives an overview of the workflow:

    PDF Icon Rapid Assembly and Analysis of Clinical Sequencing Data on a Desktop Computer: Using DNASTAR Software to Identify Potential Disease-Causing Mutations

     

    Request a fully-functional, free trial of Lasergene Genomics Suite.

     

     

    Lasergene Genomics Suite Exome Assembly Benchmarks
    Data Set
    		 Sequence Technology
    Reference Sequence
    Genome Size (Mbp)
    Number of Reads (M)
    Number of Bases (Mbp)
    		 Read Length (bp) Coverage

    Assembly Time**

    8 Human Exomes* (multiplex) Illumina

    Hg19+dbSNP

    		 27 1,268 12,047 76 446X 14 Hrs
    Human Exome*
    Illumina
    27
    163
    12,390
    		 76
    459X
    2 Hrs.

     

    *Data sets for these projects were obtained from NCBI's Short Read Archive.

    **Assembly times were calculated using a computer with a 4-Disk RAID-0.