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Supported Sequencing Technology: Illumina

As an Illumina user, you will find that Lasergene Genomics Suite enables you to effortlessly assemble and analyze your sequencing data for the following project types:

  • De novo genome assemblies
  • De novo transcriptome assemblies
  • Reference-guided genome assemblies
  • Targeted amplicon re-sequencing
  • Whole genome/whole exome validation
  • Large-scale, multi-sample SNP analysis
  • ChIP-Seq and miRNA analysis
  • Gene expression analysis
    		•

    Our software uses parameters that have been optimized specifically for Illumina data and provides full support for Illumina multiplexed data, merge pair data, and paired-end sequence data.

     

    Request a fully-functional, free trial of Lasergene Genomics Suite.

     

     

    Benchmarks: Illumina Reference-Guided Assembly
    Data Set
    		 Sequence Technology
    Reference Sequence
    Genome Size (Mbp)
    Number of Reads (M)
    Number of Bases (Mbp)
    		 Read Length (bp) Coverage

    Assembly Time

    Human Genome* Illumina Hg19+dbSNP 3,101 3,523 112,738 32 36X 23 Hrs.
    8 Human Exomes* (multiplex) Illumina

    Hg19+dbSNP

    		 27 1,268 12,047 76 446X 14 Hrs
    Human Exome*
    Illumina
    27
    163
    12,390
    		 76
    459X
    2 Hrs.
    Rice Genome* Illumina IRGSP build 4 + dbSNP 382 272 8,708 32 23X 1.5 Hrs.
    Arabidopsis Genome* Illumina TAIR10 120 67 5,000 75 42X 1 Hr.
    K-12 E. coli Genome*
    Illumina
    MG1655
    5
    2.5
    250
    100
    50X
    1 Min.
    "Deep" K-12 E. coli Genome*
    Illumina
    MG1655
    5
    45
    4,539
    100
    908X
    28 Min.
    ChIP- Seq (3 human samples)
    Illumina
    3,101
    106 M
    1,273
    36
    NA
    1.5 Hrs.
    RNA-Seq (6 human samples) Data provided by Illumina.
    Illumina
    3,101
    322 M
    2,687
    50
    NA
    3.5 Hrs.

     

    Benchmarks: Illumina De Novo Assembly
    Data Set
    Sequence Technology
    Number of Reads (K)
    Number of Bases (M)
    Coverage

    Assembly Time

    K-12 E. coli Genome*
    Illumina
    2,500
    250
    		51X
    100
    17 Min.
    "Deep" K-12 E. coli Genome* Illumina 10,000 4,539 205X
    82
    		 1.5 Hrs.

    *Data sets for these projects were obtained from NCBI's Short Read Archive.

    **All projects completed on a desktop computer that Dell sells for < $3,000.