As an Illumina user, you will find that Lasergene Genomics Suite enables you to effortlessly assemble and analyze your sequencing data for the following project types:
- De novo genome assemblies
- De novo transcriptome assemblies
- Reference-guided genome alignments
- Targeted amplicon re-sequencing
- Assembly and analysis of gene panel data
- Whole genome/whole exome validation
- Large-scale, multi-sample SNP analysis
- ChIP-Seq and miRNA analysis
- Gene expression analysis
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Our software uses parameters that have been optimized specifically for Illumina data and provides full support for Illumina multiplexed data, merge pair data, and paired-end sequence data.
The white paper below provides a comparison of Illumina gene panel and whole exome assembly results from two industry-leading pipelines: the BWA read-mapper in combination with the Broad Institute’s Genome Analysis Toolkit (GATK) Unified Genotyper variant caller, and the BWA read-mapper in combination with the GATK Haplotype Base Caller.
Results demonstrate that SeqMan NGen 12.2 (part of Lasergene Genomics Suite) achieves better sensitivity and increased coverage compared to the BWA+GATK workflows. SeqMan NGen 12.2 also assembles the data and performs variant calling three times faster than the BWA+GATK pipelines. The results from this comparison show that Seqman NGen is a fast, high accuracy read-mapper/variant caller for Illumina sequencing data.
Through Illumina's BaseSpace® environment, DNASTAR now offers two applications. First, DNASTAR offers temporary access to fully functional versions of SeqMan NGen and SeqMan Pro. Together, these two programs allow you to assemble your Illumina reads on the DNASTAR Cloud and then view and analyze the results. Second, DNASTAR features a free application to quickly assemble a de novo bacterial genome in SeqMan NGen. For a brief overview of these applications, watch our video in the Illumina BaseSpace® Webinar Series.