SeqMan NGen Logo
SeqMan NGen Rated #1 for Assembling Illumina and 454 Data

SeqMan NGen is groundbreaking sequence assembly software that has the ability to assemble any size genome quickly and accurately on a desktop computer. How quickly? Check out our benchmarking data. SeqMan NGen assembles data from all major next-gen sequencing platforms, and provides an extremely easy-to-use interface that steps you through your sequence assembly and analysis project. SeqMan NGen is fully integrated with Lasergene's SeqMan Pro, so that once your assembly is complete, you can continue with downstream analysis including discovering SNPs using Bayesian statistical models, identifying large insertions and deletions, evaluating coverage, and annotating your consensus sequence. To learn more, see the Features or Resources tab below.

 

"SeqMan NGen is a very powerful application; a 454 run can be assembled to a reference genome in just a few minutes."

Elisabeth Navarro, CNRS

Features

Supported Workflows

  • Reference-guided assemblies for any size genome
  • De novo genome and transcriptome assemblies
  • Targeted re-sequencing/exome assemblies to identify low frequency SNPs and variants.
  • Metagenome and population assemblies and variant analysis
  • Large-scale, multi-sample SNP analysis

    •  

    Fast and Accurate Assembler

  • Utilize our proprietary algorithms for fast and accurate assemblies on a desktop computer. See our benchmarking data for more information.
  • Take advantage of optimized assembly parameters for each of your projects, as well as the freedom to make adjustments if desired.Video See it!
  • Use paired-end sequence information during assembly, as well as for downstream analysis and contig ordering.
  • Assemble multiple samples against a single reference simultaneously for post-assembly SNP comparison between individuals and groups of individuals.

    •  

    Post-Assembly Analysis

  • Perform SNP and genotype calling in haploid and diploid organisms using Bayesian statistical models Video See it!
  • Compare SNP and small indels to dbSNP, COSMIC, and GERP databases during assembly for all major model organisms Video See it!
  • Utilize the structural variant report for identifying large insertions and deletions in reference-guided assemblies
  • Analyze coverage depth, group contigs into scaffolds, and annotate your consensus Video See it!

    •  

    Input/output Files

  • Import data from all major next-gen and third-gen platforms, including Illumina, Roche 454, Pacific Biosciences, SOLiD, and Ion Torrent.
  • Input multiplexed samples using standardized tag sets or user-defined custom tags.
  • Export as BAM, SAM, or ACE files.
  • Export your project to GenVision for a publication-quality graphical image of the assembly.

    • Resources

    Try It

    Try SeqMan NGen for Free!

    Request a fully functional free trial of SeqMan NGen to try it out for yourself.

     

    Free Trial for Windows

    Free Trial for Macintosh

    Free Trial for Linux - CentOS

    Free Trial for Linux - Ubuntu


    Buy It

    Ready to Buy?

    To purchase SeqMan NGen, please fill out our brief quote request form. A member of our dedicated sales team will respond to you within one business day.