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Supported Sequencing Technology: Ion Torrent

Lasergene Genomics Suite seamlessly assembles and analyzes Ion Torrent sequencing data, making it the perfect complement for Ion Torrent users. Our software runs at unprecedented speed, even on low-cost desktop computers, enabling researchers to prepare a sample, generate sequence data, and assemble and analyze their data in a single day. DNASTAR Lasergene software supports the following Ion Torrent project types:

  • De novo genome assemblies
  • De novo transcriptome assemblies
  • Reference-guided genome assemblies
  • Targeted amplicon re-sequencing, including
    support for the Ion AmpliSeq™ Cancer Panel
  • Whole Genome/Whole Exome Validation
  • ChIP-Seq, RNA-Seq and miRNA analysis
    		•

    Our software uses parameters that have been optimized specifically for Ion Torrent data and provides full support Ion Torrent barcoded data, Ion Torrent mate pair and paired-end sequence data and has recently significantly enhanced variant call accuracy in Ion Torrent data assemblies. 

     

    "The software has been very simple to use. The DNASTAR team is working closely with Life Technologies to simplify the software for the everyday scientist to perform quick and efficient sequence analysis of genes of interest. I’m able to plug raw sequence data from the Ion Torrent server (i.e., FASTQ files) into the software suite and perform SNP analysis, multiple sequence alignments and phylogenetic trees. If I can do this type of bioinformatics, anyone can."

    Dr. Luke Daum, co-founder of Longhorn Vaccines & Diagnostics, Inc.

     

    Benchmarks: Ion Torrent Reference-Guided Assembly
    Data Set
    Genome Size
    		Sequence Technology
    Number of Reads
    Number of Bases
    		Coverage

    Assembly Time*

    		Gapped?
    K-12 E. coli Genome 4.6 Mbp Ion Torrent 2 M 150 M 35X 1 Min. Y
    Ion AmpliSeq™ Cancer Panel 3,095 Mbp Ion Torrent 1 M 85 M >1,000X 22 Min. Y

     

    Benchmarks: Ion Torrent De Novo Assembly
    Data Set
    		 Sequence Technology
    Number of Reads
    Number of Bases
    		Coverage Contig N50Minimum contig size where at least 50% of all the sequence data is represented

    Assembly Time*

    K-12 E. coli Genome Ion Torrent 2 M 150 M 32X 33 Kbp 24 Min.

    *All projects completed on a desktop computer that Dell sells for < $3,000.