After saving a help topic in PDF format, why do I see overlapping text and/or unexpected symbols?

This issue only occurs in Windows. If you see overlapping text and/or unexpected symbols after saving a Lasergene help topic as an Adobe PDF document, you may need to change your Adobe settings:


  1. Click Start > Devices and Printers.
  2. Double-click on Adobe PDF.
  3. Choose Printer > Properties.
  4. In the General tab, click on the Preferences button. Uncheck Rely on system fonts only; do not use document fonts. Click Apply.
  5. In the Paper/Quality tab, click on the Advanced button. Click the link Substitute with Device Font. From the drop-down menu, choose Download as SoftFont. Double-click on PostScript Options, and click the link Automatic. From the drop-down menu, select Native TrueType.
  6. Click OK, then Apply, then OK.
  7. Open the Lasergene help topic of interest and save it as an Adobe PDF document.
  8. Open the newly-saved document. The text should now display legibly.


The instructions above apply specifically to Windows 7, but other Windows operating systems use a similar procedure.


How do I change the percent of licenses that can be checked out as commuter licenses?

Commuter licenses can be checked out for an extended period of time. Therefore you may want to limit how many licenses can be checked out as commuter licenses. You will need to indicate a certain percentage that can be used as commuter licenses in environment variables.


For Windows XP/Windows 2003:



  1. Open the Control Panel (Classic View) and go to System.
  2. Select the Advanced tab.
  3. Click on the Environmental Variables button.

For Windows Vista/Windows 7/Window 8/Windows Server 2008/Windows Server 2012:



  1. Open the Control Panel and go to the category System and Maintenance (or System and Security) > System.
  2. Select Advanced System Settings and then click on the Environmental Variables button.
  3. Double click on LSERVOPTS in the System variables list. This variable value will contain -l followed by the path to the log file.
  4. After the path, enter a space, then -com, followed by another space, then a 2-digit number indicating the percent. For example, -com 50 would indicate 50% of all licenses can be used by commuter licenses.
  5. Restart the computer for the change to take effect.

For Macintosh:



  1. Locate the file Hard Drive:Library:DNASTARLicenseServer:com.dnastar.license-server.plist.
  2. Drag the com.dnastar.license-server.plist script file onto a text editor icon such as TextEdit or BBEdit.
  3. Locate the line: /Library/Logs/DNASTARLicenseServer.log
  4. After the path, enter a space, then -com, followed by another space, then a 2-digit number indicating the percent. For example, -com 50 would indicate 50% of all licenses can be used by commuter licenses: /Library/Logs/DNASTARLicenseServer.log -com 50
  5. Save the file back as a plain text file (not rich-format).
  6. Restart the computer for the change to take effect.


How do I find the custom enzyme and selector files in SeqBuilder?

After editing an enzyme file or enzyme selector file in SeqBuilder, the revised file is saved in a different location from the original file. To display the path to the newly-saved file, use the Help > About SeqBuilder (Windows) or SeqBuilder > About SeqBuilder (Macintosh) commands.

What are the technical requirement for running SeqMan NGen?

Requirements vary based on the size and type of assembly (de novo vs. templated). See the Lasergene Genomics Suite  Technical Requirements page for detailed information.

How do I set up a license server and client?


For detailed, illustrated instructions on setting up a license server and client, please consult the DNASTAR Lasergene Network Setup Guide.

How do I check out a license on the network?

If you are using a network version of Lasergene, you can check out a license using the Commuter License Manager. This manager is installed on each client machine in the same directory as the Lasergene or ArrayStar applications.


Users may use the Commuter License Manager to check out one simultaneous use of a license for up to thirty days. This checked-out license decrements the overall available licenses for all users. Once a license is checked out, the client computer can be disconnected from the network and the client applications will continue to function.


A commuter license is useful if you have Lasergene or ArrayStar on a laptop and check out a license to use the laptop in a remote location. Remember to check the license back in when returning so that others can utilize it.


The percent of licenses that can be checked out as commuter licenses can be changed, if desired. By default, 100% of all licenses not in use can be checked out. Click here for more information on changing the percentage of available commuter licenses.


Note: We do not recommend checking out a license on a one-user network, as this will take up the entire license and prevent others from using it.

Where does Lasergene install its data files?

In the following file paths, note that ‘x’ represents the software version number.


Most Lasergene data files are installed in the following directory:


  • Windows XP: C:\Documents and Settings\All Users\Shared Documents\DNASTAR\Lasergene ‘x’ Data
  • Windows Vista, Windows 7 and Windows 8: C:\Users\Public\Public Documents\DNASTAR\Lasergene ‘x’ Data
  • Macintosh: Hard Drive:Applications:DNASTAR:Lasergene ‘x’ Data
    Included in this directory are the genetic codes, SeqBuilder’s cloning vector catalog, the vector data used in SeqMan, Borodovsky matrices and matrix patterns for GeneQuest, the repetitive and contaminant sequences folders for SeqMan, layout templates for SeqBuilder, default outlines for Protean and GeneQuest, as well as demo data for the Lasergene Core Suite Tutorials.


Enzyme and enzyme selector files are installed in the Lasergene parent directory:


  • Windows: C:\Program Files\DNASTAR\Lasergene ‘x’
  • Macintosh: Hard Drive:Applications:DNASTAR:Lasergene ‘x’

Note that once an enzyme file or enzyme selector file has been edited, the revised file is saved in a different location:


  • Windows XP: C:\Documents and Settings\All Users\Application Data\DNASTAR\DataManager\1.0
  • Windows Vista, Windows 7 and Windows 8: C:\Program Data\DNASTAR\DataManager\1.0
  • Macintosh: Hard Drive:Library:Preferences:DNASTAR:DataManager:1.0 or Hard Drive: Applications:DNASTAR:Lasergene ‘x’
    This path for the revised files can be viewed by using the Help > About SeqBuilder (Windows) or SeqBuilder > About SeqBuilder (Macintosh) commands.


Miscellaneous Lasergene and installation-related files are listed with their locations in the following PDF document:


A table of file locations is located in the “File Locations” section at the end of the Standalone Installation Guide.


Note: The installer for Lasergene does not transfer data files from any earlier version’s directories. To use customized support files with Lasergene, copy the files to the corresponding Lasergene directories. We recommend that you back up any customized support files when uninstalling older versions of Lasergene.

I purchased a perpetual license from DNASTAR. What rights do I have and what obligations does DNASTAR have under this license?

In the normal course of business, DNASTAR frequently grants the user of our software a perpetual license for the use of such software. A perpetual license is defined as the use of the license for the software version purchased, for an unlimited period of time. A perpetual license does not permit users to place DNASTAR’s software on more computers than were licensed for use in the initial purchase.


DNASTAR is not under any obligation to provide users of the software with support or additional copies of the original version of software purchased for backup, upgrades to new computers, or for any other purpose. In addition, DNASTAR is under no obligation to update its software for new computer operating systems, hardware configurations or for any other reason after initial purchase of the license by the customer. Users may continue to use a particular software version after DNASTAR support has been discontinued as long as the use of such software is not in conflict with any more recent license agreement agreed upon by the customer and only on the number of computers originally covered in the purchase agreement with DNASTAR or one of its distributors or agents.

I want to install DNASTAR applications on several computers. What options are available?

DNASTAR applications can be licensed to run on multiple computers by purchasing a site license, network license, or multiple standalone licenses. Lasergene, SeqMan NGen, and ArrayStar can be purchased with any of the three options, while GenVision is available in the standalone or site license versions.


To learn about the benefits of each option, please refer to our Software Licensing Options page.  In addition, a member of our Sales team can discuss the various licensing alternatives with you and assist in determining the optimal license type for your situation.


Can DNASTAR do my assembly for me?

We’d be happy to! DNASTAR can custom assemble, analyze and deliver a finished genome, or part of a genome, along with customized visualizations and reports. In collaboration with our sister company, Scarab Genomics, we can even prepare the library, arrange for sequencing, and then assemble and analyze the resultant data.


For more information on any of these services, see DNASTAR’s Genome Assembly Services page.


How can I get help with my own specific project?

Our experienced, on-site Support team can assist you at any stage of your project by phone or e-mail. You can also request that Support set up a free, one-on-one training webinar to walk you through your project.


How do I learn how to use the software? Are there any tutorials?

DNASTAR offers a vast and constantly-updated library of video tutorials, written tutorials and webinar recordings. Click one of these links to access our online learning resources:


  • Nearly 30 archived webinars, 1-hour online seminars covering a particular workflow in detail.

How do I reference the software in publications?

Citations for DNASTAR software should follow the format:

<Application Name with corresponding trademark>. Version <version number>. DNASTAR. Madison, WI.

For example:

SeqMan NGen®. Version 12.0. DNASTAR. Madison, WI.

To find the appropriate trademark for a DNASTAR application, please refer to the “Trademarks” section of our Legal Information page. For examples of recent publications that cited DNASTAR software, see our Published References page.

Can I publish with the free trial software?

Unfortunately, is not permissible to publish data or images obtained using DNASTAR’s free trial software. For more information, refer to the license agreement displayed during installation of the free trial software. Note that DNASTAR’s Marketing department routinely tracks citations and references to our software in the literature.


To purchase the software so that you can legally publish data and images (including those generated while you were using the free trial version), choose either of the following options:


  • Contact our Sales and Support staff by e-mailing support@dnastar.com or by calling 866-511-5090 in the U.S. and Canada. For European and UK contact numbers, please see our Contact Us page.

What are the latest improvements to the software and how can I obtain them?

To see a list of the most recent software features, see our Product Updates page.


To upgrade to the most recent version of the software, choose either of the following options: 

How can I see which version is currently released and which version I’m running?

To see which version number is the most current, see our Product Updates page.


To learn which version of an application you are running:


  1. Launch the DNASTAR application.
  2.  Select Help > About [AppName] on Windows, or [AppName] > About [AppName] on Macintosh to open a pop-up window showing the version number of the application.

How can I tell which DNASTAR products I own?

To see a list of DNASTAR products that you have purchased:


  1. Go to the DNASTAR log in page.
  2. Enter your Email/User ID and Password to log into your account.


You will be taken to a page listing your purchased applications and their version numbers, along with the number of remaining keys and the expiration dates for service plans.


An informal way to see which products have been installed/authorized is to launch the DNASTAR Navigator application. Applications listed in color are installed/authorized, and those that are “grayed out” are not. Note that this procedure does not differentiate between purchased versions of the applications and free trial versions. If you have installed a free trial version, it is best to use the numbered steps, above.


  1. Dear DNASTar
    Somebody design DNA sequence for me to send for a company to synthesis but he did not explain our sequence completely . If it is possible please my sequence in detail for me.

    Thank you very much

  2. I was very pleased when I received the first letter from a reputable Dnastara!
    I heartily thank you for your interest in us – a scientist. Soon we will meet again. I congratulate the friendly team with 30-year anniversary. Congratulations dear friends! I wish you all good health, happiness, longevity, greater success in work and life!
    Sincerely: Doctor of Chemical Sciences Sokhrab Ismailov

  3. When saving my files in genebank format (.gb) SeqBuilder says I will lose layout information if doing so (rather than saving files as .sbd). What is this layout information? What will I lose exactly?

    Thank you very much in advance for your help.
    You can as well answer to my email address (mentioned above) if you prefer.

  4. Hi Julien,

    The layout information this dialog refers to includes active views, colors, and elements visible on the SeqBuilder views. These items are not supported in the GenBank format. If you do wish to retain the SeqBuilder layout, you can use Save As and save the file as a SeqBuilder (.sbd) document.

    More information can be found in our online help: http://www.dnastar.com/t-help-seqbuilder.aspx. You may also contact us at any time at support@dnastar.com.


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