SeqBuilder
» Printable PDF overview for SeqBuilder
» View a video tutorial of SeqBuilder
Sequence editing, mapping, annotation, and automated virtual cloning
SeqBuilder enables you to edit nucleic acid and amino acid sequences and view the sequences and related items in multiple ways. SeqBuilder's window can be split into multiple panes so that more than one view can be displayed at any time. The possible views include a residue-specific view, a linear map, a circular map, a compact map of restriction cut sites (mini map), as well as a list of features, a summary of all restriction cut sites, and any comments. Each view lists a number of items that can be individually turned on or off including features, restriction sites, translations in all six reading frames, ORFs, and rulers. Many of these items can also be positioned and modified to emphasize certain characteristics. Dynamic links between the sequence and its annotations automatically update feature coordinates when you edit a sequence. Available functionality also includes the ability to reverse complement, translate, back-translate sequences, identify ORFs and perform BLAST and Entrez text* searches directly to NCBI or your personal DNASTAR StarBlast Server (sold separately).
SeqBuilder's editing capabilities are also integrated into many other Lasergene modules. Edits made in SeqBuilder will be shared automatically with other Lasergene applications that are analyzing the same data, making SeqBuilder an integral member of the powerful Lasergene suite.
> SeqBuilder Features
- Automated Virtual Cloning
- Create a New Cloning Project by adding (cut and paste) the insert you wish to clone
- Select the method you wish to follow and the software will provide the appropriate vector selection from the Lasergene Vector Catalog
- Add your own Vectors to the Vector Catalog
- The Cloning Wizard will provide help if the ends to be cloned are not compatible
- The wizard will construct the Virtual clone automatically once the ends and the vector selected are correct
- Flexible User Interface and Graphic Display
- View sequences in a variety of forms including graphically rich circular and linear displays
- Split the window to display multiple views of the same sequence simultaneously
- Customize graphical and textual displays through simple curtain and click-and-drag interface
- Create, design and move graphic annotations
- Create default displays for specific types of annotations
- View double-stranded or single-stranded DNA, RNA or amino acid sequences
- Create single page maps or multi-page posters
- Interpret and display Genbank features graphically
- Display lists of features, summary of cut sites and sequence comments
- Display and save concise summaries of restriction sites sorted by classification
- Copy and paste high-quality, resizable pictures into other software applications
- Integration with other Lasergene applications
- Share sequence and feature edits simultaneously across multiple Lasergene modules
- Open and save project files from GeneQuest, MegAlign, Protean and PrimerSelect
- Editing and Analysis
- Create or edit constructs through standard editing and enhanced copy/paste functions
- View ORFs that are updated with every sequence edit
- Translate DNA or back translate protein using any of the supplied genetic codes or customize your own
- Choose restriction sites to display based on any combination of:
- Manual selection of enzymes
- Frequency of cuts
- Type or class of enzyme
- Site complexity
- Overhang compatibility
- Find related sequences using the integrated BLAST and Entrez* search tools
- Edit the sequence, then the feature coordinates will update automatically
- Sequence Entry
- Import data from many popular file formats
- Batch import Vector NTI files retaining feature names
- Export data into the original file format automatically when the file format is one of several exportable formats offered
- Download sequences directly from NCBI* or a DNASTAR StarBlast database using accession numbers, BLAST, or text searches
* Requires Internet Connection
» Printable PDF overview for SeqBuilder
» View a video tutorial of SeqBuilder
Manage vectors, inserts, clones, and other sequences in project files
Automated virtual cloning for three protocols
View the sequence, ORFs, translations
Restriction site summary view and enzyme manager
Alter the display in many views, like this linear view
Restriction site summary view and enzyme manager
Display as a circular map