Choose Assembly Workflow

The Choose Assembly Workflow dialog lets you select from a variety of workflow types divided into categories.

 

 

Once you make a selection, SeqMan NGen will populate the rest of the wizard with appropriate default parameters for your project. For example, your selection in this screen determines which assembly types are enabled in the next screen, entitled Choose Assembly Type, as shown in the table below.

 

This selection in Choose Assembly Workflow…

…enables these options in Choose Assembly Type

Reference based – normal workflows

Reference based with host removal

De Novo

De Novo with host removal

Reference-guided with gap closure

Reference-guided – special workflows

Variant Calling

Sanger Validation

Whole genome

x

 

x

 

x

x

 

x

Exome and gene Panel

x

 

 

 

 

 

x

x

Transcriptome/RNA-Seq

x

 

x

 

 

 

 

 

ChIP-Seq

x

 

x

 

 

 

 

 

miRNA

x

 

x

 

 

 

 

 

Metagenomics/population assembly

x

x

x

x

 

 

 

 

Viral-host integration

x

 

 

 

 

 

 

 

Merge existing assemblies from same or different workflows

(NA – See Combine and/or Reanalyze Assemblies)

Rerun analysis of existing assemblies

(NA – See Combine and/or Reanalyze Assemblies)

 

Once you are finished, click Next > to continue to the next wizard screen.