DNASTAR Next-Gen Sequencing Software Solutions


Reference-Guided Assembly with Automated Gap Closure

DNASTAR's Lasergene Genomics Suite now includes software that automates the process of gap closure following a genomic assembly. This functionality greatly reduces the need for manual finishing of your assembled project to address gaps caused by structural variants, such as insertions and deletions. Simply specify your paired-end sequence reads and a related reference sequence, and let the software do the following automatically:

  • Assemble pair-end reads to the reference sequence, and identify structural variants present
  • Split reference-guided assembly at structural variant coordinates
  • Remove reference genome bias and auto-assemble novel regions, resulting in a scaffolded assembly with most structural variants resolved
  • Assemble unmapped reads de novo producing contigs that can be used to close scaffold gaps
  • For more information on using Lasergene Genomics Suite for automated gap closure, please see the following video, which demonstrates an assembly showing a reduction in the number of unresolved structural variants from 31 using our standard workflow to four using this new workflow, dramatically reducing the work required for de novo genome closure.


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