Supported Sequencing Technology: Roche 454

Lasergene Genomics Suite supports accurate, fast assembly and comprehensive analysis of Roche 454 sequencing data for the following project types:

De novo genome assemblies De novo transcriptome assemblies Reference-guided genome assemblies Targeted re-sequencing Large-scale, multi-sample SNP analysis Metagenome and population assemblies and variant analysis

Our software uses assembly and trimming parameters that have been optimized specifically for Roche 454 data and provides full support for Roche 454 multiplexed data.

Request a fully-functional, free trial of Lasergene Genomics Suite.

Benchmarks: Roche 454 Reference-Guided Assembly

Benchmarks: Roche 454 De Novo Assembly

Data Set	Sequence Technology	Number of Reads (K)	Number of Bases (M)	Coverage	ContigN50 (Kbp)Minimum contig size where at least 50% of all the sequence data is represented	Assembly Time
K-12 E. coli Genome*	Roche 454	1,075	606	30X	26	18 Min.
Rodent Transcriptome*	Roche 454	1,064	570	16X	1,199	5.5 Hrs.

*Data sets for these projects were obtained from NCBI's Short Read Archive.

**All projects completed on a desktop computer that Dell sells for < $3,000.

PRODUCT INFORMATION

VIDEOS

Watch one of our videos that demonstrate the simplicity of using our software to assemble and analyze your Roche 454 data.

	Roche 454: De Novo Bacterial Assembly (3:49)
	Roche 454: De Novo Transcriptome Assembly (2:47)

»See more next-gen videos