Supported Sequencing Technology: Roche 454

Lasergene Genomics Suite supports accurate, fast assembly and comprehensive analysis of Roche 454 sequencing data for the following project types:

De novo genome assemblies De novo transcriptome assemblies Reference-guided genome assemblies Targeted re-sequencing Large-scale, multi-sample SNP analysis Metagenome and population assemblies and variant analysis

Our software uses assembly and trimming parameters that have been optimized specifically for Roche 454 data and provides full support for Roche 454 multiplexed data.

 

Benchmarks: Roche 454 Reference-Guided Assembly

Data Set	Genome Size	Sequence Technology	Number of Reads	Number of Bases	Coverage	Assembly Time*	Gapped?
Yeast Genome	12 Mbp	Roche 454	1 M	311 M	25X	3 Min.	Y
Human Genome	3,095 Mbp	Roche 454	74 M	20,000 M	6X	6 Hrs.	Y

 

Benchmarks: Roche 454 De Novo Assembly

Data Set	Sequence Technology	Number of Reads	Number of Bases	Coverage	Contig N50Minimum contig size where at least 50% of all the sequence data is represented	Assembly Time*
K-12 E. coli Genome	Roche 454	385 K	69 M	18X	56	22 Min.
Rodent Transcriptome	Roche 454	1 M	570 M`	NA	NA	2.5 Hrs.

*All projects completed on a desktop computer that Dell sells for < $3,000.

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