PrimerSelect's comprehensive set of tools enables you to design and analyze primers for PCR, sequencing, probe hybridization and transcription. Enter your own primers or let PrimerSelect generate a sorted list of suggested primers for all or part of your DNA, RNA or back-translated protein template. You can accept PrimerSelect's top choice, evaluated on the thermodynamic properties for annealing reactions, or view all of the options and choose alternatives based on your specialized knowledge. PrimerSelect includes the WorkBench where you preview the effects your choices will have on reading frames and restriction sites. PrimerSelect further assists you by highlighting potential pitfalls in both standard and multiplex PCR experiments via illustrations, graphs and statistical reports. These tools enable you to limit investment in time-consuming and costly experiments to those that have the highest probability of success. PrimerSelect even lets you perform BLAST or Entrez text searches and load sequences directly from NCBI's databases*.
PrimerSelect Features
- Sequence Entry
- Import data from many popular file formats
- Read sequences and features from other Lasergene project files
- Download sequences directly from NCBI* using accession numbers, BLAST, or text searches
- Customize Primer Design and Editing
- Adjust parameters such as primer and product length, primer locations, Tm, hairpins or use the default settings
- Edit template sequence and share newly created features through integration with SeqBuilder and other Lasergene applications
- Determine the best primer pairs or best probes for varied conditions
- Analyze existing primers
- Create degenerate, inosine-containing and "bestguess" primers from ambiguous templates
- Utilize any of the 14 genetic codes supplied or create your own
- View the effects on restriction sites and translations when editing primer sequences
- Select from a list of alternative codons for silent mutations or specific residue changes
- Graphical and Tabular Displays
- Generate color-coded, graphical overviews of primer options and their locations
- Choose an overview of primers located on your template or view the sequence at base level with graphs of Tm and DG on both strands
- See major and alternate PCR product sizes simultaneously
- View sequences and structures of hairpins and primer-dimers
- View primers with restriction maps, reading frames and translations
- Analyze tables of amplification details, primer base compositions, primer lengths, Tm and DG.
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» Printable PDF overview for PrimerSelect
Add restriction sites or make edits to primers in the workbench
Select a primer pair from the alternatives provided