DNASTAR Next-Gen Sequencing Software Solutions

 

Clinical Research

DNASTAR's Lasergene Genomics Suite includes software for clinical research, enabling you to identify and compare potential disease-causing mutations across multiple samples or groups of samples. Features of the Lasergene Genomics Suite software include:

  • Quick assembly of large data sets from all major next-gen technologies on a desktop computer - check out our benchmarks below.
  • Detection and analysis of SNPs using live, interactive views and comprehensive filtering tools
  • Direct connection to the dbSNP, COSMIC and GERP databases
  • The ability to perform large-scale comparisons of SNPs across individuals and groups of indivduals, and determine the level of disruption to each gene caused by the SNPs present
  • For more information on using Lasergene Genomics Suite in your clinical research, please see the following video which briefly demonstrates the alignment of exome data from 10 individuals with Kabuki syndrome and 8 control individuals against the entire human genome, as well as post-assembly analysis of SNPs. For more details on this project, please see the poster below.

     

    PDF Icon Rapid Assembly and Analysis of Clinical Sequencing Data on a Desktop Computer: Using DNASTAR Software to Identify Potential Disease-Causing Mutations

     

    Request a fully-functional, free trial of Lasergene Genomics Suite.

     

     

    Lasergene Genomics Suite Benchmarks for Human Reference-Guided Assemblies

    Data Set
    Sequence Technology
    Reference Sequence
    Genome Size (Mbp)
    Number of Reads (M)
    Number of Bases (Mbp)
    Read Length (bp) Coverage

    Assembly Time**

    Human Genome* Illumina Hg19+dbSNP 3,101 3,523 112,738 32 36X 23 Hrs.
    8 Human Exomes* (multiplex) Illumina

    Hg19+dbSNP

    27 1,268 12,047 76 446X 14 Hrs
    Human Exome*
    Illumina
    27
    163
    12,390
    76
    459X
    2 Hrs.
    Ion AmpliSeq™ Cancer Panel (3 multiplexed data
    sets) Data provided by Ion Torrent.
    Ion Torrent
    3,101
    3
    99
    85 500X
    24 Min.
    Fluidigm® Access Array System (2 multiplexed data
    sets) Data provided by Pacific Biosciences.
    Pacific Biosciences Fluidigm human amplicons 0.1 < 1 7 180 500X 1 Min.

     

    *Data sets for these projects were obtained from NCBI's Short Read Archive.

    **Assembly times were calculated using a computer with a 4-Disk RAID-0.