By Matthew Keyser De novo assembly of whole transcriptome sequencing data from non-model organisms can be very challenging for a variety of reasons. Factors such as computing limitations and unseen problems lurking in the sequence data can quickly derail your results and your timeline.  For example, the presence of untrimmed Illumina adapter sequences, ribosomal RNA, and […]

Lasergene Genomics Suite now includes access to the Variant Annotation Database (VAD) for human sequencing data. I recently spoke with DNASTAR Scientist, Dr. Tim Durfee about the VAD to get a better understanding of how the tool works and how it can help genomics and clinical researchers with their variant analysis. Can you describe what […]

In our latest DNASTAR LabViews interview, we talk with Dr. Robab Katani of Penn State about her work with Escherichia coli O157:H7 strains isolated from super-shedder cattle. Watch our interview below to learn about the biological significance of these E. coli strains and super shedder cattle, and hear how DNASTAR software has helped Dr. Katani assemble and visualize multiple E. coli […]

We are excited to announce our newest video series, DNASTAR LabViews, a collection of short interviews with DNASTAR users. Our first interview is with Dr. Xiao-Ning Zhang at St. Bonaventure University. Watch the video below to learn about Dr. Zhang’s RNA splicing research, and hear how her lab uses DNASTAR Lasergene for genomics, transcriptomics, and […]

Users often ask how much RAM is required for a de novo genome assembly in Lasergene Genomics Suite, and we addressed that question for Lasergene 12 users in an earlier blog post. We’re excited to announce that with the release of Lasergene 13, RAM requirements for de novo genome assembly are significantly decreased, with the […]

In a previous post we described the RAM dependencies for de novo genome assemblies, which showed a linear relationship with genome size. Extrapolation for assembling large, eukaryotic genomes would suggest RAM requirements measured in terabytes, but quite often de novo assembly is not the goal. Rather, you wish to align large numbers of reads against […]

DNASTAR recently released a series of white papers that compare alignment and variant calling for next-gen sequence data in Lasergene Genomics Suite to some of our commercial and open source competitors. While these papers primarily focus on accuracy and computational speed, there is a third set of metrics that is more challenging to measure: What […]

  Last week, DNASTAR attended the American Society of Human Genetics Annual Meeting along with nearly 8,000 other attendees. While at the conference, we had the opportunity to meet with many researchers in the fields of human genetics, genomics and clinical research, including some of our existing customers as well as those who are new […]

If you are using Next Generation Sequencing (NGS) for clinical research, cancer genomics, genome-wide association studies, or other genomic research, the ability to identify variants with confidence is of utmost importance. For these projects, you need software that can correctly identify the true variants, while minimizing false positives that could lead to wasted research effort. […]

You’ve prepped your samples, submitted them for sequencing, and finally received a file with your sequence data. Now what? It’s time to assemble, align, and analyze your data and start to make good on that investment you just made! Maybe you already have software for this purpose, or maybe you are evaluating new software to […]