Date: 6/12/17   Version Affected: 14.1 and earlier   Version Fixed: 14.1.2   Issue: Users running Windows machines with an active non-English language pack or with certain non-American region settings may experience issues (e.g. stalling or errors during assembly), particularly with RNA-Seq or ChIP-Seq workflows in SeqMan NGen.  These issues are partially related to the […]

Date: 6/12/17   Version Affected: 14.1 and earlier   Version Fixed: 14.1.2   Issue: Users running Windows machines with an active non-English language pack or with certain non-American region settings may experience issues (e.g. stalling or errors during assembly), particularly with RNA-Seq or ChIP-Seq workflows in SeqMan NGen.  These issues are partially related to the […]

Date: 5/5/2017   Version Affected: 13.1 to 14.1   Version Fixed: NA   Issue: The Variant Annotation Database (VAD) based on human genome reference GRCh37 was first released with Lasergene 13.1 in March 2016. With the March 2017 release of  Lasergene 14.1,  a companion VAD version was added, based on human genome reference GRCh38. DNASTAR […]

Date: 3/2/17   Version Affected: 14.1 and later   Version Fixed: N/A   Issue: As of March 2, 2017, corresponding with the release of Lasergene 14.1, DNASTAR no longer supports SeqMan NGen for the Linux operating system. We will continue to support SeqMan NGen for Windows and Macintosh.   Resolution: If you have questions about […]

Date: 4/26/16   Version Affected: 13.0.0    Version Fixed: 13.0.1/13.02 included in Lasergene 13.0c installer   Issue: In SeqMan NGen version 13.0.0, the number of targeted aligned reads is not properly reported due to an issue handling duplicate reads. This issue can be seen in the Enrichment Report section of the Coverage of Target Regions Report in SeqMan […]

Date: 4/7/16   Version Affected: 13.0   Version Fixed: 13.0.1   Issue: In SeqMan NGen v. 13.0, the Viral-Host Integration workflow may lead to an unexpectedly low number of reads aligning to the host genome. This issue has been addressed with the release of a patch, Lasergene v. 13.0.1.   Resolution: To address this issue, […]

Date: 4/7/16   Version Affected: 13.0   Version Fixed: 13.0.1   Issue: Due to a non-optimal background parameter setting in SeqMan NGen v. 13.0, the transcript annotation workflow may produce an unexpectedly low number of identified transcripts. This issue only affects the transcript annotation (de novo Transcriptome/RNA-Seq) workflow. This issue has been addressed with the […]

Date: 3/1/16   Version Affected: 12.3 and earlier   Version Fixed: N/A   Issue: As of the Lasergene 13.0 release, SeqMan NGen will no longer detect quality scores in pre-2012 Illumina data by default.   Resolution: Starting with the Lasergene 13.0 release (March 2016), SeqMan NGen uses a standard offset for Illumina data rather than basing […]

Date:   7/28/2014   Version Affected:  12.0 and earlier   Version Fixed:  12.0.1   Issue:  Rarely, specific reference sequences or genome templates may cause SeqMan NGen to crash during the assembly phase.   Resolution:   Crashes have been reported to occur when using particular GenBank (*.gbk) or General Feature Format (*.gff) files, and for the Arabidopsis 138 template. However, it […]

Date: 4/20/2013   Version Affected:  11.0 and later   Version Fixed:  N/A   Issue: When a SeqMan NGen assembly uses all available memory, the connection to the DNASTAR Cloud may be lost without warning.   Resolution:   This has been observed on Safari and Google Chrome browsers, but may affect other browser types as well. If […]