Note regarding Variant Annotation Databases based on GRCh37 and GRCh38
Version Affected: 13.1 to 14.1
Version Fixed: NA
Issue: The Variant Annotation Database (VAD) based on human genome reference GRCh37 was first released with Lasergene 13.1 in March 2016. With the March 2017 release of Lasergene 14.1, a companion VAD version was added, based on human genome reference GRCh38. DNASTAR software automatically detects which version of the human genome reference sequence is used in a SeqMan NGen assembly and accesses the corresponding VAD for variant annotation.
The aforementioned Lasergene 14.1 release also included an update of the GRCh37 version of the VAD and the removal of Polyphen2 data from both VAD versions due to licensing issues.
In May 2017, to address some data inconsistencies caused by changes in the reference sequence between the GRCh37 and GRCh38 VAD builds, the GRCh37 version was reverted to its pre-Lasergene 14.1 state. We also anticipate some cases in which annotations from the GRCh38 version of the VAD will be missing, due to the changes in the reference sequence.
Resolution: DNASTAR will continue to update the VAD periodically and will strive to address any remaining inconsistencies between the two versions. In the interim, there may be cases where the data and annotations do not align as expected. For questions regarding this topic, please contact DNASTAR at 866-511-5090 or email@example.com.