The Overview provides a way to navigate within the sequences, no matter what type of sequences or alignment is used. In the case of genomic alignments generated by Mauve, the Overview also allows you to explore the relationships between multiple aligned blocks. Selections and vertical ordering within this view are synchronized with other views in MegAlign Pro. Similarly, selections and ordering done elsewhere are reflected in the Overview.
To access the view, click on the Overview tab.
By default, the Overview displays a header, followed by one or more sequences with labels to their left. Each sequence is depicted as a “sequence/alignment block,” and the length is proportional to other sequences in the group. Each sequence row can display a variety of optional data tracks.
The Overview displays information in different ways, depending on the alignment stage and the workflow. In all cases, sequences are represented as either gray or colored blocks. Any sequence that is not part of an alignment is shown in solid gray. Sequences included in an alignment are usually drawn in dark green, although multi-block alignments, such as those generated by Mauve, are drawn in up to seven colors.
See the following topics for images and details of the Overview in different situations:
The following table describes ways to control the appearance of the Overview:
|To change settings for this view||To change settings, including showing/hiding connecting lines and changing spacing between rows, use the Overview section of the Style panel.|
|To set an unselected alignment block in the Overview as the active block||Double-click on an inactive alignment block, or right-click on an inactive alignment block and choose Set As Active Block. The corresponding sequence blocks will line up vertically. The alignment block containing the sequence block will become active.|
|To change settings related to the Overview||use the Overview section of the Style panel.|
|To make an alignment block the active block and move it to the left of the Overview||Make a block active by double-clicking a sequence block. Then choose Overview > Set Start of Sequences to Active Block or the context (right-click) option of the same name. The blocks in each sequence are rotated so that the active sequence block is leftmost.|
|To center the Overview display over the active block||Choose Overview > Show Active Block.|
|To return alignment blocks to their default order||Choose Overview > Reset Start of Sequences or the context (right-click) option of the same name. In the Overview, the individual sequence ruler coordinates are updated to reflect the change.Note that resetting the start of sequences presumes that alignment consists of circular genomic sequences.|
Need more help with this?