The BLAST Search wizard is used to search a query sequence against one of NCBI’s databases. The sequence can be part or all of an active sequence, another sequence file on your computer, or typed/pasted-in text. The wizard consists of four consecutive screens, each described in a separate topic:

To launch the BLAST Search wizard, use one of the following commands or tools. Some commands let you launch the wizard with the workflow pre-selected and the Search list already pre-populated.

Command, shortcut or tool Description
Search > Sequence Search Launches the wizard at the Query screen.
Use the “Change search strategy” tool () from the
Text, Table or Pairwise views
Search > Sequence Search with Selection Launches the wizard at the Query screen, whose table is pre-populated with the currently-selected portion of sequence.
Press Ctrl/Cmd+B
Search > Sequence Search with All Sequences Launches the wizard at the Query screen, whose table is pre-populated with all sequences in the document.

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