Search - User Guide to SeqBuilder Pro

Welcome to SeqBuilder Pro

SeqBuilder Pro is Lasergene’s application for sequence editing and map creation, as well as plasmid auto-annotation, automated virtual cloning and primer design. Easily navigate between synchronized views with a click of a button, and rearrange views and toolbars…

Primers

SeqBuilder Pro lets you design primers for regions of interest on your sequence. The following video is a brief overview of SeqBuilder Pro’s primer design workflow. To learn more about working with primers, see: Create primer pairs automatically Create…

Plasmid Auto-Annotation

SeqBuilder Pro’s built-in plasmid Feature Library contains over 230 curated features, and allows you to add your own features, as well. If your vector sequences lack some annotations, or contain incomplete or obsolete versions of annotations, you can…

Windows, Views, Panels and Tools

The Document window opens when you open a nucleotide or protein sequence or a SeqBuilder (.sbd) document. This is the window where you can edit the sequence or add/edit features, restriction enzymes, ORFs, translations and/or rulers to the sequence. On the left of…

Document window views

Windows, Views, Panels and Tools » Document window views

The following video contains a short tour of each of the Document window views: To change from one view to another: Click the tab with the name of the desired view. By default, the upper portion of the window lets you choose any of 1-4 views, depending…

Automated Virtual Cloning

SeqBuilder Pro supports automated virtual cloning using a wide variety of cloning techniques. Simply select your insert and vector sequences, and SeqBuilder Pro will automatically construct the virtual clone. Choose vectors from the vector catalog provided, or add your…

Translations

SeqBuilder Pro lets you view the translation for part or all of an open nucleotide sequence or create a new protein sequence based on the translation of a nucleotide sequence. To display translations for an open nucleotide sequence: In the Sequence or Primer Design…

Step 6 (optional): Verify the clone

Automated Virtual Cloning » Step 6 (optional): Verify the clone

SeqBuilder Pro 15.1 and later offers the ability to verify whether or not a clone produced in the laboratory matches the version designed in SeqBuilder Pro. To learn how, watch this brief video or follow the written instructions below: To verify a clone: Use…

Create a New Sequence

The following video is a brief overview of sequence creation and editing in SeqBuilder Pro. To create a new DNA or protein sequence from scratch: Choose the appropriate menu command or keyboard shortcut. DNA: File > New DNA or Ctrl/Cmd+N. Protein:…

Details panel

Windows, Views, Panels and Tools » Document window panels » Details panel

The Details panel, represented by a blue “information” icon ( ) displays information about an active view or selection in the Document window. The Details panel can be opened by clicking on the Details tab. If the tab is hidden, reveal it by clicking the Show/Hide…

Agarose Gel Simulation

Agarose gel simulation lets you find the location of restriction enzymes on a gel and perform ‘mock’ digests to determine the expected size of digest products on an electrophoresis gel. Gel simulations let you find the ideal percent agarose and set of molecular…