For exome, gene panel, cancer, or genome-wide association study (GWAS) studies, the following wizard selections are generally appropriate:
- In the Choose Assembly Workflow screen, choose Exome and gene panel.
- In the Choose Assembly Type screen, select Reference based assembly.
- In the *Input Reference Sequences” page, specify a human genome.
- For cancer samples, check the box next to Somatic/Cancer/Heterogeneous in the Assembly Options screen.
In most cases, downstream analysis of the finished assembly will place in ArrayStar.
These two brief videos both demonstrate the reference-guided exome workflow:
The following video uses GWAS data. Note that the video shows an earlier version of the Project Type screen with an option called Exome. In more recent versions of SeqMan NGen, this option is called Exome and gene panel.
Need more help with this?