Post Assembly Options

The Post Assembly Options screen lets you enter a reference sequence against which to order and orient assembled contigs. In some workflows, you can also use this screen to set requirements for minimum contig size. Different subsets of options are available depending on the workflow.

• Check the box if you wish to specify Minimum contig size requirements. This option is not available for long-read workflows. If you check the box, you will need to enter the desired values for:
  
  
  • Min(imum) sequences – The minimum allowed number of sequences that make up a valid contig.
    
    
  • Min(imum) length -The size of the minimum allowed contig length for a contig to be included in the assembly.

• Check the box if you wish to Use an existing reference sequence to put assembled contigs in order. If you check this box, you will need to add at least one reference sequence using the various Add buttons in the lower half of the screen. To learn how to do this, see:
  
  
  • Add a reference sequence from your computer or the Cloud
    
  • Add a genome template from DNASTAR
    
  • Remove a sequence from the list

Click Next > to proceed to the next wizard screen or < Back to return to the previous screen.