SeqMan NGen Tutorials

The tutorials listed below all launch from SeqMan Ultra and end with analysis in SeqMan Ultra. The tutorials are available in both this User Guide and the SeqMan Ultra User Guide. Within each tutorial is a link for downloading the corresponding data in archived (.zip) format.

• Whole genome reference-guided workflow
• Whole genome de novo workflow with mate pair data
• Analysis of a whole genome de novo assembly
• De novo assembly using Sanger data
• RNA-Seq de novo transcriptome workflow

Tutorials that end with analysis in any other application are listed below, and are found only in this SeqMan NGen User Guide. Within each tutorial is a link for downloading the corresponding data in archived (.zip) format.

• RNA-Seq reference-guided workflow with analysis in ArrayStar
• ChIP-Seq workflow with analysis in ArrayStar
• Copy number variation (CNV) workflow with analysis in ArrayStar and GenVision Pro
• Whole-genome reference-guided workflow with analysis in ArrayStar |
• Long read analysis with accuracy evaluation

*Note: SeqMan NGen went through a major update with Lasergene 17.0, which was released in early 2020. Therefore, there are two sets of tutorials and tutorial data. The set included in this version of the User Guide is for Lasergene version 17.0 and later. If you are using Lasergene version 16.0 or earlier, you will want to switch to that version of the User Guide before starting the tutorials. To switch, just use the drop-down menu right above the topic name (“SeqMan NGen Tutorials”) and choose the User Guide that corresponds with your version of Lasergene.