Part C: Adding the translation step

To add a translation step after the reverse complement step:

1. Click on the pencil icon () in the outer box (white background) and select *Translate. The translation step is added and encompasses the reverse complement step and the source sequence.

2. Use the Translation table drop-down menu to select NCBI: 11 (Bacterial, Archaeal and Plant Plastid Code).

3. In the Save Results As textbox, change the name to translated int Gene. Click Run.

4. Open the output file in SeqBuilder Pro or in a suitable text editor. It should appear as below.

When SeqNinja translates a feature from a sub-region of a GenBank file, or a .seq file with GenBank style annotations, any overlapping feature (or portion of a feature) is also translated if the reading frame is not specified. The second sequence below is a translation of a piece of the gene xis that overlaps int and is translated in frame 1 instead of the native frame 3 used for xis.

This is the end of Tutorial 3.