DNASTAR Lasergene Next-Gen Sequencing Software
Roche 454 Sequencing Software

 

Supported Sequencing Technology: Roche 454

Lasergene Genomics Suite supports accurate, fast assembly and comprehensive analysis of Roche 454 sequencing data for the following project types:

  • De novo genome assemblies
  • De novo transcriptome assemblies
  • Reference-guided genome assemblies
  • Targeted re-sequencing
  • Large-scale, multi-sample SNP analysis
  • Metagenome and population assemblies and variant analysis
  • Our software uses assembly and trimming parameters that have been optimized specifically for Roche 454 data and provides full support for Roche 454 multiplexed data.

     

    Request a fully-functional, free trial of Lasergene Genomics Suite.

     

     

    Benchmarks: Roche 454 Reference-Guided Assembly
    Data Set
    Sequence Technology
    Reference Sequence
    Genome Size (Mbp)
    Number of Reads (M)
    Number of Bases (Mbp)
    Read Length (bp) Coverage

    Assembly Time

    Aspergillus Genome* Roche 454
    ASM265v1
    29
    2
    1,055
    450 36X
    38 Min
    K-12 E. coli Genome*
    Roche 454
    MG1655
    5
    1.5 M
    606
    400
    121X
    3 Min.

     

    Benchmarks: Roche 454 De Novo Assembly
    Data Set
    Sequence Technology
    Number of Reads (K)
    Number of Bases (M)
    Coverage

    Assembly Time

    K-12 E. coli Genome*
    Roche 454
    1,075
    606
    30X
    26
    18 Min.
    Rodent Transcriptome*
    Roche 454
    1,064
    570
    16X
    1,199
    5.5 Hrs.

     

    *Data sets for these projects were obtained from NCBI's Short Read Archive.

    **All projects completed on a desktop computer that Dell sells for < $3,000.