SeqMan NGen has four similar but differently-named wizard screens used to add files other than the experimental sequence files or contaminant sequences. A version of one of these screens, Input Reference Sequences, is shown below as an example.
Depending on choices made in the Choose Assembly Workflow and Choose Assembly Type screens, you will encounter zero to two of these screens, each with different names and purposes.
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Name of wizard screen: | |||
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Input Reference Sequences |
Input Host Files |
Input Viral Genomes |
Input Biome Genomes | |
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Screen description (right)
Workflow (below) |
Reference sequence(s) |
Host sequence(s) |
Viral genome sequence(s) |
Reference sequence(s) from a 16S rRNA database (e.g., Silva, Greengenes Ribosomal Database Project) |
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Only if ‘host removal’ was chosen in Choose Assembly Type |
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Only if ‘reference based was chosen in Choose Assembly Type | |
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Always |
Always |
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All others* |
Only if ‘reference’ was chosen in Choose Assembly Type |
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If any of these four screens appears, you must input the specified sequence(s) or assemblies before proceeding further in the wizard. See our Supported File Types page for allowable file extensions.
For information about performing specific tasks within this wizard screen, see the links below:
•Automatically downloading and adding genome template packages
•Automatically downloading and adding genomes from NCBI.
•Adding and removing accessory files (VCF, BED, etc.)
•Using templated Transcriptome/RNA-Seq workflow options
Once you are finished making choices in this screen, click Next > to continue to the next wizard screen.