SeqBuilder Pro contains representatives of a wide variety of restriction sites for available enzyme isotypes. By default, Lasergene’s enzyme and enzyme selector files are installed in the following directory:

  • Windows: C:\Program Files (x86)\DNASTAR\Lasergene x
  • Macintosh: Hard Drive:Applications:DNASTAR:Lasergene x

Once an enzyme file or enzyme selector file has been edited, the revised file is saved to:

  • Windows: C:\Program Files (x86)\DNASTAR\DataManager\1.0
  • Macintosh (depends on version):

    • Hard Drive:Library:Preferences:DNASTAR:DataManager:1.0

    • Hard Drive: Users:<user ID>:Library: Preferences: DNASTAR: DataManager: 1.0

    • Hard Drive: Applications:DNASTAR:Lasergene x

At any time, you may see what enzyme or selector file SeqBuilder Pro is using by selecting Help > About SeqBuilder Pro (Windows) or SeqBuilder Pro > About SeqBuilder Pro (Macintosh).

If the enzyme file is write-protected, a copy of the file will be made in a user-specified location. This copy will track changes made to enzymes, new enzymes, and deleted enzymes. Re-installing Lasergene will not overwrite the changes, but likewise this file will not receive updated enzyme definitions.

For vendor information and a list of abbreviations used by SeqBuilder Pro, see the REBASE Suppliers web page.

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