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SeqMan NGen uses a patented algorithm that provides fast and accurate sequence assembly using traditional, next-generation, and third-generation technologies. Optimized assembly parameter settings are automatically determined for you, based on your read technology and your specific project objective, such as targeted resequencing, assembly of a small genome, and de novo transcriptome workflows. SeqMan NGen is fully integrated with Lasergene's SeqMan Pro, so that once your assembly is complete, numerous tools are available for analyzing your project, including discovering SNPs, evaluating coverage, and annotating your consensus sequence. To learn more, see the Features or Resources tab below.


Features

Next-Gen Workflows Supported

  • Support of de novo genome, transcriptome, and metagenome fields of study.
  • Whole genome re-sequencing with gap closure, SNP analysis, and annotation.
  • Targeted re-sequencing of candidate genes or regions at high accuracy to identify low frequency SNPs and variants.
  • Assembly of ChIP-Seq and RNA-Seq reads against a reference genome following analysis in QSeq and SeqMan Pro.

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    Fast and Accurate Assembler

  • Utilize paired-end sequence information during assembly, as well as for downstream analysis and contig ordering.
  • Perform a hybrid assembly from any combination of next-generation sequencing, third generation, and Sanger methods.Video See it!
  • Take advantage of our optimized algorithms for fast and accurate assemblies on a desktop computer.

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    Flexible Settings and Analysis

  • Parameters are adjustable for multiple workflows. After choosing your data type, optimized parameter settings are determined for you, but can be easily modified as desired. Video See it!
  • Decide on a number of preprocessing options, including vector and end-trimming, marking known repeats, and excluding contaminant sequences such as primer reads from your assembly.
  • Analyze your finished assembly in SeqMan Pro for SNPs, coverage depth, grouping contigs into scaffolds and annotating your project. Video See it!

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    Input/output Files

  • Import data from all major next-gen and third-gen platforms.
  • Export as ACE for analysis in Lasergene or other software.Video See it!
  • Export your project to GenVision for a publication-quality graphical image of the assembly.

    • Resources

    Resources

    Whether you are a long time customer, or are just starting to learn about SeqMan NGen, you may find some of the following resources helpful:

    Technical Requirements

    Licensing Options

    Assistance in Grant Preparation

    Training Videos:

    Basic Project Setup for Next-Gen Sequence Assembly

    Analyzing SNPs and Annotating the Consensus in a Reference-Guided Next-Gen Sequence Assembly

    Solutions:

    Reference-Guided Assembly

    De Novo Assembly

    SNP Analysis in "Deep" Assemblies

    De Novo Transcriptome

    Product Literature:

    Software Solutions for Next-Gen and Third-Gen Sequencing Technologies

    Next-Generation Sequencing...and Beyond Brochure

    Understanding Transcriptomes Solutions Using DNASTAR Software

    Human Genome Scale Assembly Poster

    Assembly and Annotation of Bacterial Genomes in Your Own Lab

    SeqMan NGen Installation Guide

    SeqMan NGen Tutorials

    SeqMan NGen Tutorial Data

     

    For additional resources, please see our Support page.

     

     

    Try It

    Try SeqMan NGen for Free!

    Request a fully functional free trial of SeqMan NGen to try it out for yourself.

     

    Free Trial for Windows

    Free Trial for Macintosh


    Buy It

    Ready to Buy?

    To purchase SeqMan NGen, please fill out our brief quote request form. Request a quote for SeqMan NGen only, or add other DNASTAR products at a multiple pricing discount.