What file types can I use to create a plasmid map?
SeqBuilder Pro supports virtually any type of sequence file, including FASTA, ABI, and GenBank formats, and easily imports files from Vector NTI, SnapGene, and Geneious. For a full list of supported file types, see our Fil/e Formats resource.
How can I control which restriction enzymes are displayed on my plasmid map?
The Enzymes panel lets you control which enzymes are displayed on your plasmid map. You can choose to apply groups of enzymes based on the frequency of cuts, type or class, site complexity, overhang compatibility, or an intersection of any of these groups. You can also choose to show/hide individual enzymes from the Enzymes panel by simply checking or un-checking the box next to its name.
How do I move the restriction enzyme labels on my plasmid map?
To move a restriction enzyme label on your plasmid map, first click on the label to select it. Then, click and drag the label to the desired location. Other enzyme labels on your plasmid map will be moved automatically to avoid overlapping.
Can I control which annotations are displayed on my plasmid map?
Yes. To show/hide features on your plasmid map, open the Features view. Check the rows for the features you want to display, and remove the check mark for the features you want to hide. As you do this, your plasmid map will update automatically.
Can I import annotations for my plasmid map?
Yes. If your plasmid sequence lacks some annotations, or contains incomplete or obsolete versions of annotations, you can effortlessly annotate them using SeqBuilder Pro’s built-in curated Feature Library. To do this, open your plasmid map and select Features>Annotate Sequence. Accept the default parameters or edit if desired, then click OK. The Annotation Results window will appear, displaying features in the Feature Library that matched the specified parameters. Review and select the features you’d like to add, and then click Accept Checked to automatically add the annotations to your sequence.
Can I customize the Feature Library used for auto-annotating my plasmids?
Yes. You can add your own annotations to the curated Feature Library that comes installed with SeqBuilder Pro in one of two ways:
- From the circular map, linear map, or sequence view, click on a feature to select it, then choose Features > Send to Feature Library.
- Open the Feature Library Manager by selecting Features>Feature Library Manager, then drag and drop a sequence from your computer’s file explorer into the library window. Any features included in the sequence will be extracted and saved as features in Feature Library database.
How do I change the appearance of the annotations on my plasmid map?
To change the appearance of a feature, first select it on your plasmid map. Then, access the Style panel to change the rendering style of your selected feature, including the color, shape, label position, and more.
To change the feature style for all of a certain type of feature, go to the Features panel. Right-click on the type of feature you wish to edit, and choose Select All Features With This Type. Then, edit the rendering style as desired from the Style panel. The changes you make will be applied to all of the features with the type you selected.
How do I export my plasmid maps for publication?
To save your plasmid map as an image, first make sure the circular map is the active view, then select Edit>Copy as Picture. This copies the image to your clipboard to be pasted into another application. When pasting the copied picture into a Microsoft Office application, we recommend using Edit>Paste Special and selecting Picture (Enhanced Metafile).
