Lasergene Molecular Biology is a comprehensive package of sequence analysis software, for DNA, RNA, and protein sequence data. This package includes all the tools you need to perform multiple and pairwise sequence alignments, identify and annotate genes, assemble Sanger sequences, create virtual clones, design primers, concatenate sequences, find a reverse complement, perform an NCBI BLAST search, and more.
Lasergene Molecular Biology provides comprehensive cloning software. Simulate clones with confidence using our highly accurate and easy-to-use software. For cloning projects, simply select your insert and vector sequences and restriction sites, and SeqBuilder Pro will automatically design PCR primers, recognize recombination sites, identify compatible restriction sites and construct the virtual clone for you.
Lasergene Molecular Biology provides comprehensive primer design software. Create PCR primers and primers for PCR-based cloning methods, as well as sequencing primers that can improve coverage or extend into gaps in a de novo assembly.
All of the sequence editing, annotation, cloning and primer design workflows listed above can be completed in SeqBuilder Pro, a component of Lasergene Molecular Biology which can be purchased online as a single application for as little as $99/year for an academic license.
SeqBuilder Pro, part of Lasergene Molecular Biology, allows you to edit DNA, RNA, and amino acid sequences quickly and easily. Take your analysis to the next level with batch editing functions such as reverse translation and extraction of GenBank style features. The built-in analysis tools allow you to simulate restriction enzyme digests, BLAST gene and protein sequences, and more!
Lasergene Molecular Biology provides comprehensive support for assembly and analysis of Sanger sequencing data within the SeqMan Pro application. SeqMan Pro allows you to assemble your read data de novo or against one or more reference sequences. You can then use the analysis tools within the application to view read alignment and coverage, assess SNPs and annotate the consensus sequence.
White papers:
♦ Accuracy of de novo assembly of Sanger trace data: SeqMan Pro vs. three alternative pipelines
The MegAlign Pro application in Lasergene Molecular Biology provides DNA, RNA, and protein sequence alignment using a range of pairwise and multiple aligning methods. From the sequence alignments generated, you can quickly create phylogenetic trees as well as reports and tables.
Lasergene Molecular Biology provides automated annotation of plasmids and other sequences, using matches to a curated feature database, as well as gene discovery and annotation tools for novel sequences. Take advantage of our expertly curated database of common plasmid vector features and identify feature matches automatically with SeqBuilder Pro. The software also includes applications for identifying ORFs, spliced junctions, transcription factor binding sites and restriction sites, and for the identification of regions that match user defined DNA or protein sequences.
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