Make your clone virtually before making it in the lab

• Supports Gateway, Gateway Multisite, Gibson, GeneArt, In-Fusion, TA, TOPO, or PCR-directed restriction enzyme molecular cloning methods
• Choose vector sequence(s) from the built-in  catalog or use a custom vector
• View step-by-step instructions for preparing the vector and amplifying the insert
• View details about fragment inserts, primers, fragment junctions
• Save or print instructions for making the clone in your lab
• Export publication-quality images

Prevent costly errors

• Align Sanger reads to validate insert alignment and orientation of sequenced clones
• Check and validate your plasmid insertion
• Catch read misalignment and insertion errors

Are your plasmid features out-of-date?

• Auto-annotate your plasmids with accurate, up-to-date features from our curated plasmid vector database.
• Specify  feature matching stringency parameters
• Once matches are found, update all  features to the latest version automatically or make choices individually
• Sortable and interactive annotation results table
• Add your custom features to the feature library
• Create colorful, customizable plasmid maps

Primers that work… the FIRST time!

• Create primers for PCR-based cloning; to increase coverage or extend into gaps in a de novo assembly
• Utilize our PCR site-directed mutagenesis workflow, which enables you to see the impact of a sequence mutation on the structure of a protein before designing your primers
• Select a range of interest guided by feature and ORF information
• Specify default or custom primer locations, conditions, melting temperature and more
• View major and alternate primer pairs simultaneously
• Automatically check for mispriming (dimers and hairpins)
• Easily modify primers by length, restriction sites, or codon mutations
• Create and share primer catalogs

See the structural impact of a mutation before designing your primers

• Determine desired variants by using Lasergene Protein to scan your protein for “hot-spots” to identify residues that contribute to fold stability, or create amino acid variants and predict protein structural changes
• Design and mutate PCR primers to introduce desired variants
• Create expression clones to amplify mutated gene using PCR-based cloning methods

Sequence Editing

Many customizable display options

• Translate DNA, back-translate protein, reverse complement DNA, and more with manual or automated sequence editing options
• Apply reading frames and view restriction enzyme binding sites
• Search for and open sequence matches from NCBI’s BLAST database
• Use a text query to search for and open sequences from NCBI’s Entrez database
• Create and edit GenBank style features
• Create plasmid maps and export publication-quality graphics

Agarose Gel Simulation

Skip the mess with virtual gels!

• Plot DNA fragment mobility in silico
• Choose from a huge selection of commercial gels or create a custom gel
• Add enzymes, primers and/or molecular weight markers
• Watch “hours” pass in a fraction of a second!
• Compare your virtual gel with the image simulation to confirm complex banding patterns