Lasergene 19.0.1 Release Notes

Lasergene 19.0.1 is now available to download. This is a minor release for Lasergene 19.0 and introduces several enhancements in Lasergene Protein (Figure 1).

• ESMFold2 structure prediction is available to NovaLocal and NovaCloud users: The ESMFold2 algorithm has been added as a protein structure prediction option alongside Boltz and AlphaFold-Multimer (Figure 2). ESMFold2 uses a protein language model to predict accurate 3D structures directly from an amino acid sequence without requiring a multiple sequence alignment (MSA), enabling GPU-accelerated predictions in seconds for proteins under 1,000 amino acids. It is particularly well suited for protein-protein and antibody-antigen complex prediction.

• AlphaFold-Multimer can now be run using NovaLocal, as well as NovaCloud: AlphaFold-Multimer predictions can now be run locally through NovaLocal. During setup, users can choose to automatically download either the reduced or full AlphaFold reference database (Figure 3).

• Two new MMseqs2 server options for NovaLocal users running Boltz: NovaLocal now supports two MMseqs2 server configurations for Boltz predictions. Each is available as a premium add-on to your NovaLocal license. The DNASTAR-hosted MMseqs2 server requires no setup, retains no user data, supports queued multi-sample predictions, and is ideal for multi-user environments (Figure 4). Alternatively, a locally hosted MMseqs2 server can be deployed using a new setup tool in DNASTAR Navigator. Once configured, the local server requires no Internet connection and keeps all data on your computer. This option is best suited for single-user workflows.

• Histidine scanning has been added to Protein Design: Protein Design now supports Histidine scanning in addition to the existing Alanine and Serine scanning methods. Histidine scanning is particularly useful for identifying catalytically important residues and potential transition metal-binding sites.

• Improved protein prediction setup feedback: Protean 3D’s protein prediction setup wizard now provides clearer feedback and guidance during prediction configuration. It can also detect prediction jobs that are likely to exceed available system resources and warns users before the prediction begins.

• Dynamic delivery of future protein prediction technologies: NovaCloud and NovaLocal users can now receive selected future protein structure prediction technologies and updates without reinstalling Protean 3D, making it easier to access new capabilities as they become available.

Recommended action:

We recommend that all users update to Lasergene 19.0.1 to benefit from these fixes.

Figure 1: Protean 3D, the flagship application of Lasergene Protein.

Figure 2: Available protein modeling prediction algorithms now include ESMFold2.

Figure 3: AlphaFold-Multimer can now be run locally. In addition, you control the size of the database to download.

Figure 4: When running Boltz locally, you can choose DNASTAR’s secure MMseqs2 server as an alternative to Colabfold’s server.