Use SeqBuilder Pro to confirm the accuracy of your clone created in the lab.
You’ve finished creating your clone in the lab and are ready to move on to downstream experiments, but how do you know that all your steps have resulted in the expected recombinant clones? Many researchers verify their clone accuracy through Sanger sequencing. We make it easy to perform clone sequence verification to confirm that your Sanger sequencing results perfectly match the clone you designed. Our clone sequence verification workflow confirms that the clone fully contains the insert of interest in the correct orientation, is in-frame with the vector, and confirms that there is adequate coverage of trace files across the entire clone. If discrepancies are identified during the clone sequence verification process, we show you exactly where the disagreements lie, so that you can address them. Use SeqBuilder Pro’s clone sequence verification workflow to catch read misalignment and insertion errors before proceeding with downstream experiments.