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Lasergene 19.0 Release Notes

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      • Sequence Editing and Annotation
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      • Antibody Phage Display
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      • Protein Structure Prediction
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SEQMAN ULTRA

Accurately trim, assemble, evaluate, and edit Sanger and NGS sequencing data.

SeqMan Ultra

SeqMan Ultra is a modern, intuitive sequence assembly application built on the foundation of our classic SeqMan Pro. SeqMan Ultra uses the most accurate algorithm available for Sanger sequence assemblies and provides extensive post-assembly analysis tools for both Sanger and NGS assemblies. When compared to the competition, our algorithm does the best job at assembling Sanger sequences into a single contig and calling the most accurate consensus sequence based on trace quality and shape.

SeqMan Ultra supports multi-sample Sanger assembly and gives you the flexibility to choose between a classic SeqMan Pro-style workflow or a genomics-driven approach.

SeqMan Ultra

Assemble multi-sample Sanger data directly in SeqMan Ultra using the classic SeqMan Pro algorithm and a user interface similar to that in SeqMan Pro. Or run multi-sample assemblies with SeqMan NGen to align Sanger data to human chromosomes and access advanced variant analysis tools like the Variant Annotation Database (available to Lasergene Genomics customers).

Following assembly, SeqMan Ultra uniquely offers you the ability to easily edit your assembled data—exposing or trimming ends, introducing mutations—as well as analyze your assembled results through chromatogram viewing, coverage evaluation, variant analysis, and multi-sample comparison within a single contig. With SeqMan Ultra, you can move seamlessly between classic Sanger workflows and advanced genomics-scale assemblies—all within a single, intuitive platform designed to deliver accuracy, flexibility, and control.

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SeqMan Ultra-Related Workflows

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Sanger Sequence Assembly

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Clinical Research

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De Novo Genome Assembly

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Metagenomic Assembly

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Variant Analysis

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Viral Genome Analysis

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Whole Genome/Exome Sequencing

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Local BLAST Searching in Lasergene

Resources

Please see our resources below for more information on SeqMan Ultra.

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Comprehensive Variant Analysis: from Raw Sequence Data to Aligned Genomes

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De novo assembly and analysis of Sanger/ABI data

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Sanger Sequence Assembly in SeqMan Ultra

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De Novo Sanger Data Assembly in SeqMan Ultra

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Dr. Michael Pauly of Mapp Biopharmaceutical on Ebola and ZMapp

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SeqMan Ultra Help

User Guide

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SeqMan Ultra Tutorials

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FAQs

What’s the difference between SeqMan NGen, SeqMan Ultra, and SeqMan Pro?

SeqMan NGen and SeqMan Ultra are two applications that work together to assemble and analyze both Sanger and NGS sequencing data. SeqMan NGen is the…

SeqMan NGen and SeqMan Ultra are two applications that work together to assemble and analyze both Sanger and NGS sequencing data. SeqMan NGen is the application that assembles and aligns the data, and is fully integrated in SeqMan Ultra so that you can go from streamlined project setup to finished assembly all in one application. SeqMan Ultra is one of the sequence assembly tools that allows you to analyze and edit the assembly produced by SeqMan NGen. (Additional applications within Lasergene Genomics are available for analyzing NGS projects and more complex Sanger assemblies).

SeqMan Ultra is the new face of SeqMan Pro, our longstanding contig assembly software application, complete with a new modern, intuitive interface, as well as lots of performance improvements under the hood. SeqMan Ultra is a 64-bit application, which means when compared to the 32-bit SeqMan Pro application, it offers faster project opening, better performance for analyzing large files, increased capacity, and compatibility with macOS 10.15. SeqMan Pro will still be available in Lasergene 17, but will eventually be completely replaced by SeqMan Ultra.

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Which Lasergene package includes SeqMan Ultra?

SeqMan Ultra is included with Lasergene Molecular Biology, Lasergene Genomics, and the complete DNASTAR Lasergene package.

With a Lasergene Molecular Biology license, SeqMan Ultra is available for Sanger sequence assemblies only.

With a Lasergene Genomics or DNASTAR Lasergene license, SeqMan Ultra is available for all NGS assemblies as well as Sanger sequence assemblies.

Can I search NCBI databases online? Can I search local databases?

GenVision Pro, Protean 3D, SeqMan Ultra, SeqBuilder Pro, and MegAlign Pro all offer the ability to do online sequence similarity searches in NCBI BLAST and text similarity searches in NCBI Entrez. Each of these applications also let you create, manage, and search local databases. To do this, you first use the Local Database Manager to upload local files and/or import sequences from NCBI, with over 30 databases to choose from. The manager can also be used to merge, expand, rename, and/or delete local databases. Local searches can then be done without Internet access and are a great way to ensure data security. In addition, local searches are 5–25x faster than online searches.

Can I edit my assembled sequences in SeqMan Ultra?

Yes. An assembly run in SeqMan Ultra or SeqMan NGen produces a fully editable project which can be edited at base-level and contig-level in SeqMan Ultra.

Compare DNASTAR Lasergene Packages

  MOST POPULARDNASTAR Lasergene
Lasergene Molecular Biology Lasergene Genomics Lasergene Protein
Included Applications
SeqBuilder Pro
SeqMan Ultra
MegAlign Pro
GeneQuest
GenVision
SeqNinja
SeqMan NGen
ArrayStar
GenVision Pro
Protean 3D
DNASTAR Navigator
Supported Workflows
Antibody Phage Display
Automated Virtual Cloning
Clone Sequence Verification
ELN Integration
Gel Electrophoresis Simulation
Mauve Genome Alignment
Multiple Sequence Alignment
Pairwise Sequence Alignment
PCR Primer Design
PCR Site-Directed Mutagenesis
Phylogenetic Analysis
Plasmid Maps
Sequence Editing and Annotation
Sanger Sequence Assembly
ChIP-Seq Data Analysis
Clinical Research
De Novo Transcriptome Assembly
Large Scale Variant Analysis
Metagenomic Assembly
NGS De Novo Genome Assembly
RNA-Seq Alignment
Viral Genome Analysis
Whole Exome/Genome Sequencing
Antibody Modeling
Epitope Prediction
Protein Docking
Protein Sequence Analysis
Protein Stability Prediction
Protein Structural Alignment
Protein Structure Analysis
Protein Structure Prediction
Integrates with
DNASTAR Cloud Data Drive
DNASTAR Cloud Assemblies  
NovaCloud
NovaLocal
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