DNASTAR

What file types can I import for sequence editing?

SeqBuilder Pro supports  virtually any type of sequence file, including FASTA, ABI, and GenBank formats, and easily imports files from Vector NTI, SnapGene, and Geneious. For a full list of supported file types, see our File Formats resource.

How can I control which restriction enzymes are displayed on my sequence?

The Enzymes panel lets you control which enzymes are displayed on your sequence. You can choose to apply groups of enzymes based on the frequency of cuts, type or class, site complexity, overhang compatibility, or an intersection of any of these groups. You can also choose to show/hide individual enzymes from the Enzymes panel by simply checking or un-checking the box next to its name.

How do I add annotations to my sequence?

The sequence annotation tools in SeqBuilder Pro make it easy annotate your sequence. Simply select the range of sequence for your annotation and do one of the following:

• Choose Features>New Feature to create a new misc_feature.
• Choose Features>New Feature with Translation to create a new CDS feature with  a /translation qualifier attached to it.

The feature type and rendering for any feature can be changed at any point from the Features view.

Can I create reverse complements of multiple sequences simultaneously?

Yes. To reverse complement one or more sequences, choose Tools > Batch Reverse Complement. In the resulting window, add the sequences you wish to reverse complement into the Sequences folder, then click Run. The reverse complemented sequences will be displayed in the Results folder.

Can I create reverse complements of multiple sequences simultaneously?

Yes. To reverse complement one or more sequences, choose Tools > Batch Reverse Complement. In the resulting window, add the sequences you wish to reverse complement into the Sequences folder, then click Run. The reverse complemented sequences will be displayed in the Results folder.

Can I create translations of multiple sequences simultaneously?

Yes. To translate one or more nucleic acid sequences into protein sequences, choose Tools > Batch Translate. In the resulting window, add the sequences you wish to translate into the Sequences folder. Choose the reading frame and genetic code to be used for translation, then click Run. The tranlated sequences will be displayed in the Results folder.

How do I join multiple sequences together into a single sequence?

The sequence editing tools in SeqBuilder Pro make it easy to join two or more sequences end-to-end. To do this, choose Tools>Concatenate. In the resulting window, add the sequences you wish to join into the Sequences folder. Select the output file format, then click Run. The new concatenated sequence will be displayed in the Results folder.

What file types are supported for exporting my edited sequence file?

SeqBuilder Pro supports the export of many different file types, including EMBL, FASTA, and GenBank formats. For a full list of supported file types, see our File Formats resource.

Can I export an image of my sequence for publication?

Yes. Our sequence editing software allows you to save the sequence view, circular map view, or linear map view as an image for publication. First, make sure the view you want to export is active, then select Edit>Copy as Picture. This copies the image to your clipboard to be pasted into another application. When pasting the copied picture into a Microsoft Office application, we recommend using Edit>Paste Special and selecting Picture (Enhanced Metafile).