LASERGENE MOLECULAR BIOLOGY
The software solution for DNA, RNA and protein sequence alignment and analysis.
Choose from a variety of molecular cloning techniques: Gateway cloning, Gibson Assembly, GeneArt cloning, In-Fusion cloning, TA cloning, TOPO cloning and restriction enzyme cloning.
Predict coding regions and intron or exon boundaries files for splice sites, start sites and transcription factor binding sites. Align known genes, expressed sequence tags (EST) or proteins with your sequence.
Batch annotate your sequences, match plasmid features and replace existing features from the plasmid vectors database.
PCR primer and QPCR primer design software for primer analysis, primer checks, and sequencing primers with a customizable primer database. View the effect on key characteristics such as primer melting temperature or primer annealing temperature.
Sequence assembly and sequence analysis of Sanger sequencing data. Assemble data de novo or against one or more reference sequences and use dna analysis tools to view sequence alignment, SNPs analysis and annotate the consensus sequence.
DNA, RNA, and protein sequence alignment tools using a range of pairwise alignment and multiple sequence alignment methods. Use the phylogenetic tree maker from the generated sequence alignments.
Manually edit saved DNA or protein sequences or download a sequence directly from NCBI GenBank. Control and customize expression-specific genetic codes for translating and back-translating sequences, reverse complement, invert, translate and ORF identification.
Included Applications and Features
SeqBuilder Pro (formerly SeqBuilder) is DNASTAR’s software application for designing primers, virtual molecular cloning and analysis of dna sequences. Plasmid mapping software and plasmid drawing tools included.
GeneQuest is DNASTAR’s software application to locate and annotate genes, regulatory elements and structure for complete DNA sequence analysis. Display restriction sites and agarose gel separations of restriction digests. Predict intron boundaries with pattern files for the transcription factor binding site.
MegAlign Pro is DNASTAR’s software application to perform multiple sequence alignments of large DNA, RNA, or protein sequences and provides four alignment methods: MUSCLE, Mauve, MAFFT, and Clustal Omega.
GenVision is DNASTAR’s software application for genome visualization, designed to support easy generation of publication quality graphics of annotated genomes. Customize to compare gene functionality, illustrate gene expression levels or visualize coverage of an assembled contig.
SeqMan Pro is DNASTAR’s software application for sequence assembly and analysis of Sanger sequencing data with comprehensive SNP analysis, also providing variant analysis with annotation. Discover variants and annotate.
SeqNinja is DNASTAR’s software application for automated and batch editing of genome sequences, supporting a variety of file types. Generate a multi-sequence FASTA file by extracting features, generating a reverse complement dna sequence, or translating a GenBank file.
Watch the overview of Lasergene Molecular Biology to learn more about the included applications to design PCR primers, align multiple sequences, clone a gene, and more.
Essential sequence alignment software for DNA primer design, molecular cloning and complete DNA sequence analysis.
Primer Design + Molecular Cloning Software
- Support for all major cloning methods, including Gibson Assembly, InFusion, Gateway, Multisite Pro Gateway, TOPO, TA Cloning and restriction enzyme analysis
- Primer designing and data analysis software
- Create and share primers catalogs, including primers for PCR primer design
Multiple Sequence Alignment Software
- Multiple sequence alignment software solutions and pairwise sequence alignment methods including MUSCLE, MAFFT, and Clustal Omega
- Whole genome multiple alignment software using MAUVE
- Phylogenetic tree capabilities
Sanger Sequencing Software
- Assemble reads de novo, or against one or more reference sequences
- Assess reads with alignment software, coverage and SNPs
- Design sequencing primers to improve coverage
- Validate NGS data with Sanger reads
Comprehensive Sequence Analysis Software
- Accurate and fast plasmid auto-annotation and plasmid map creation
- Sequence editing, including automated and batch editing
- Gene discovery
- Integrated NCBI BLAST search
- Publication quality graphics
Looking for EditSeq, PrimerSelect, or MegAlign?
EditSeq, PrimerSelect, and MegAlign have been retired. The functionality from those applications can now be accessed using MegAlign Pro and SeqBuilder Pro.
Check out our illustrated user guide, Transitioning to SeqBuilder Pro & MegAligPro, featuring short topics showing how to do all your favorite workflows in the newer applications.