LASERGENE MOLECULAR BIOLOGY
The software solution for DNA, RNA and protein sequence alignment and analysis.
Lasergene Molecular Biology is the comprehensive sequence analysis and alignment software you need to support your molecular biology research. The package provides software solutions for DNA, RNA and protein editing and annotation, Sanger sequence assembly, multiple and pairwise sequence alignment, virtual cloning, primer design and comprehensive sequence analysis. Streamline your workflow with automated plasmid annotation and large-scale batch editing; automatically concatenate, translate, reverse complement, or extract features from multiple sequences simultaneously. Translate DNA or back translate protein using the genetic code of your choice. Find out why users worldwide recognize DNASTAR as a leading sequence alignment software for life scientists.
Choose from a variety of molecular cloning techniques: Gateway cloning, Gibson Assembly, GeneArt cloning, In-Fusion cloning, TA cloning, TOPO cloning and restriction enzyme cloning.
Use gel electrophoresis simulation to easily determine the ideal percent agarose and set of molecular weight markers to resolve your DNA fragments before running gel electrophoresis in the lab.
Effortlessly display and customize the sites and features on your plasmid map and take advantage of our built-in automated tool to accurately annotate your plasmid using our extensive, curated database of features.
PCR primer and QPCR primer design software for primer analysis, primer checks, and sequencing primers with a customizable primer database. View the effect on key characteristics such as primer melting temperature or primer annealing temperature.
Sequence assembly and sequence analysis of Sanger sequencing data. Assemble data de novo or against one or more reference sequences and use dna analysis tools to view sequence alignment, SNPs analysis and annotate the consensus sequence.
DNA, RNA, and protein sequence alignment tools using a range of pairwise alignment and multiple sequence alignment methods. Generate and compare multiple phylogenetic trees using RAxML, for Maximum Likelihood trees, or the Neighbor Joining method.
Manually edit saved DNA or protein sequences or download a sequence directly from NCBI GenBank. Control and customize expression-specific genetic codes for translating and back-translating sequences, reverse complement, invert, translate and ORF identification.
Included Applications and Features
SeqBuilder Pro (formerly SeqBuilder) is DNASTAR’s software application for designing primers, virtual molecular cloning and analysis of dna sequences. Plasmid mapping software and plasmid drawing tools included.
SeqMan Ultra is DNASTAR’s software application for analyzing and editing Sanger sequence assemblies. Easily edit your assembled data, including exposing or trimming ends, and introducing mutations, and analyze your assembled project by viewing chromatograms, evaluating coverage and analyzing variants.
GenVision is DNASTAR’s software application for genome visualization, designed to support easy generation of publication quality graphics of annotated genomes. Customize to compare gene functionality, illustrate gene expression levels or visualize coverage of an assembled contig.
MegAlign Pro is DNASTAR’s software application to perform multiple sequence alignments of large DNA, RNA, or protein sequences and provides four alignment methods: MUSCLE, Mauve, MAFFT, and Clustal Omega.
GeneQuest is DNASTAR’s software application to locate and annotate genes, regulatory elements and structure for complete DNA sequence analysis. Display restriction sites and agarose gel separations of restriction digests. Predict intron boundaries with pattern files for the transcription factor binding site.
SeqMan Pro is DNASTAR’s legacy application for Sanger sequence assembly and analysis. SeqMan Pro will still be available in Lasergene 17, but will eventually be completely replaced by SeqMan Ultra.
SeqMan NGen is DNASTAR’s software application for assembling and aligning sequencing data, and is included in Lasergene Molecular Biology for accurate assembly of your Sanger data de novo or with a reference sequence. To assemble NGS data, a Lasergene Genomics license is required.
SeqNinja is DNASTAR’s software application for automated and batch editing of genome sequences, supporting a variety of file types. Generate a multi-sequence FASTA file by extracting features, generating a reverse complement dna sequence, or translating a GenBank file.
Essential sequence alignment software for DNA primer design, molecular cloning and complete DNA sequence analysis.
Primer Design + Molecular Cloning Software
Sanger Sequencing Software
- Assemble reads de novo, or against one or more reference sequences
- Assess reads with alignment software, coverage and SNPs
- Add sequences to close gaps within a scaffold
- Validate NGS data with Sanger reads
Looking for EditSeq, PrimerSelect, or MegAlign?
EditSeq, PrimerSelect, and MegAlign have been retired. The functionality from those applications can now be accessed using MegAlign Pro and SeqBuilder Pro.
Check out our illustrated user guide, Transitioning to SeqBuilder Pro & MegAligPro, featuring short topics showing how to do all your favorite workflows in the newer applications.