SeqMan Pro |
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Lasergene’s SeqMan Pro offers quick and accurate sequence assembly and analysis of Roche 454 Next-Gen and Sanger data, in addition to providing visualizations and analysis of next-gen projects assembled by SeqMan NGen. Discover SNPs, analyze coverage, annotate your consensus and much, much more. To learn more, see the Features or Resources tabs below.
Features
Assemble Contigs
Assemble your de novo or reference-guided assembly from Roche 454 technology or Sanger data. You can also view your Illumina or third-gen sequence assembly from a SeqMan NGen assembly in SeqMan Pro.
Paired-end data support for 454, Sanger and Illumina data from a SeqMan NGen assembly to visualize the orientation and distance between contigs.
Assemble certain groups of sequences separately, each into its own contig using the Assemble in Groups feature.
Discover SNPs
Live, interactive SNP reports allow you to view information about a putative SNP while at the same time, viewing it in the context of the Alignment View.  See it!
Multiple filters enable you to control which SNPs to review. For example, you may show only SNPs that occur a certain distance from a coding feature, show only novel SNPs, or show only SNPs that occur within a certain depth of coverage. See it!
SNP Discovery parameters allow customization of the criteria SeqMan Pro uses to identify putative SNPs. See it!
Annotate your Project
Easily copy features from constituent sequences to the consensus sequence. See it!
Add features to the consensus sequence through BLAST searching. See it!
Annotate sequence files with features prior to assembly to display them in the Alignment View.
Save or export the consensus sequence to SeqBuilder to see SeqMan Pro's auto-annotation of features for areas of high and low coverage, as well as confirmed SNPs. See it!
Analyze Coverage
Use the Coverage Threshold graph to view coverage within the assembly as it compares to the threshold parameters. See it!
Review the Depth of Coverage graph to see the depth of sequences throughout the assembly. The numbers to the left of the graph indicate the actual number of sequences represented.
Choose locations to drive the closure or to fill in areas of low coverage using the Primer Walking feature. See it!
Export Data for Further Analysis
Export the consensus sequence for one or more contigs, with or without gaps and layout features (representing each constituent sequence). If desired, you may limit the export to a range of the consensus.
Export the consensus sequence to SeqBuilder for designing primers for regions of low coverage, automatically annotated by SeqMan Pro.
Export your project to GenVision for a publication-quality graphical image of the assembly.
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To purchase Lasergene, please fill out our brief quote request form. Request a quote for Lasergene only, or add other DNASTAR products at a multiple pricing discount.
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