Lasergene Genomics Suite's variant analysis software provides tools to simplify the discovery and analysis of variants in multiplexed data generated by all major next-gen sequencing platforms including Roche 454, Illumina, Ion Torrent, and Pacific Biosciences. Our variant analysis software enables you to:
- Quickly and easily assemble large data sets from all major next-gen technologies on a desktop computer—check out our benchmarks below
- Analyze variants using live, interactive views and comprehensive filtering tools
- Compare variants to dbNSFP, dbSNP, COSMIC, and GERP databases during assembly for all major model organisms
- Perform large-scale comparisons of variants across individuals and groups of indivduals, and determine the level of disruption to each gene caused by the variants present
For more information on using Lasergene Genomics Suite's variant analysis software for your large-scale, multi-sample variant projects, please see the following two videos, which demonstrate an assembly of the exomes of three individuals, and the subsequent analysis and comparision of variants across the group.
Lasergene Genomics Suite Reference-Guided Genome Alignment Benchmarks for Variant Analysis
|Human Genome*||Illumina||Hg19+dbSNP||3,101||3,523||112,738||32||36X||23 Hrs.|
|8 Human Exomes* (multiplex)||Illumina||Hg19+dbSNP||27||1,268||12,047||76||446X||14 Hrs.|
|Human Exome*||Illumina||Hg19+dbSNP||27||163||12,390||76||459X||2 Hrs.|
|Ion AmpliSeq™ Cancer Panel (3 multiplexed data sets)Data provided by Ion Torrent.||Ion Torrent||Hg19+dbSNP||3,101||3||99||85||500X||24 Min.|
|Fluidigm® Access Array System (2 multiplexed data sets)Data provided by Pacific Biosciences.||Pac Bio||Fluidigm human amplicons||0.1||< 1||7||180||500X||1 Min.|
*Data sets for these projects were obtained from NCBI's Short Read Archive.
** Assembly times were calculated using a computer with a 4 Disk RAID-0.