To add a translation step after the reverse complement step:
- Click on the pencil icon () in the outer box (white background) and select *Translate. The translation step is added and encompasses the reverse complement step and the source sequence.
- Use the Translation table drop-down menu to select NCBI: 11 (Bacterial, Archaeal and Plant Plastid Code).
- In the Save Results As textbox, change the name to translated int Gene. Click Run.
- Open the output file in SeqBuilder Pro or in a suitable text editor. It should appear as below.
When SeqNinja translates a feature from a sub-region of a GenBank file, or a .seq file with GenBank style annotations, any overlapping feature (or portion of a feature) is also translated if the reading frame is not specified. The second sequence below is a translation of a piece of the gene xis that overlaps int and is translated in frame 1 instead of the native frame 3 used for xis.
This is the end of Tutorial 3.
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