SeqMan NGen


Software for Next-Gen Sequence Assembly

The keystone of Lasergene Genomics Suite, SeqMan NGen, is groundbreaking sequence assembly software that has the ability to assemble any size genome quickly and accurately on a desktop computer. SeqMan NGen assembles data from all major next-gen sequencing platforms, and provides an extremely easy-to-use interface that steps you through your sequence assembly and analysis project. SeqMan NGen is fully integrated with SeqMan Pro, so that once your assembly is complete, you can continue with downstream analysis including discovering SNPs using Bayesian statistical models, identifying large insertions and deletions, evaluating coverage, and annotating your consensus sequence. SeqMan NGen also assembles RNA-Seq, ChIP-Seq, and miRNA data, allowing easy, integrated, post-assembly analysis in ArrayStar with QSeq of both gene expression and SNP data.





Features & Highlights

 


Assemble and Align NGS Data Quickly and Accurately


  • SeqMan NGen is >99.8% accurate for SNP prediction
  • Project parameters are automatically optimized based on data and project type
  • Multiple samples can be aligned against a single reference simultaneously for post-assembly SNP comparison between individuals and groups of individuals
  • Automated gap closure available for bacterial genome assemblies

 




Perform In-Depth Post-Assembly Analysis with Ease


  • SNP and genotype calling in haploid and diploid organisms performed using Bayesian statistical models
  • SNP and small indels compared to dbNSFP, dbSNP, COSMIC, and GERP databases during assembly for all major model organisms
  • Structural variant report available for identifying large insertions and deletions as well as small indels in reference-guided alignments
  • Depth of coverage graphs and reports, consensus annotation, and contig scaffolding offered for comprehensive project analysis and finishing

Next-Gen Software
For All Major Sequencing Technologies