SeqMan Pro


Software for Contig Assembly and Analysis, Including SNP Discovery, Coverage Evaluation, and Project Annotation

Lasergene’s SeqMan Pro offers quick and accurate sequence assembly and analysis of Sanger data, in addition to providing visualizations and analysis of next-gen projects assembled by SeqMan NGen. Discover SNPs, analyze coverage, annotate your consensus, and much more. SeqMan Pro projects can also be exported to GenVision for publication-quality graphical images of the assembly.





Features & Highlights

 


Visualization and Analysis of NGS Projects from SeqMan NGen


  • Paired-end data support for Illumina, Ion Torrent, and Roche 454 data from a SeqMan NGen assembly to visualize the orientation and distance between contigs.
  • Take advantage of comprehensive variant analysis, including a wide range of available filters, and mutliplex support, allowing you to view SNPs on a per sample basis.
  • Save selected SNPs from your project in a custom VCF SNP table for use in subsequent assemblies.
  • View coverage and RPKM values for targeted regions in exome capture projects.
  • Utilize the structural variant report for identifying large insertions and deletions, as well as small indels, in reference-guided assemblies
  • Export SNP data to ArrayStar for large-scale SNP comparisons and gene-impact analysis between multiple individuals and groups


 




Sanger Sequence Assembly and Analysis


  • Assemble Sanger sequences de novo or with a reference sequence.
  • Utilize paired-end data, if desired, to visualize the orientation and distance between contigs.
  • Use the Primer Walking feature to drive closure or to fill in areas of low coverage.
  • Live, interactive SNP reports allow you to view information about a putative SNP while at the same time, viewing it in the context of the Alignment View.
  • Annotate your consensus in a variety of ways, including adding features from a BLAST search.