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SeqMan NGen Rated #1 for Assembling Illumina and 454 Data

SeqMan NGen 3 represents a breakthrough in sequence assembly software by being able to assemble any size genome quickly and accurately on a desktop computer. How quickly? Check out our benchmarking data. SeqMan NGen assembles data from all major next-gen sequencing platforms, and provides an extremely easy-to-use interface that steps you through your sequence assembly and analysis project. SeqMan NGen is fully integrated with Lasergene's SeqMan Pro, so that once your assembly is complete, you can continue with downstream analysis including discovering SNPs using Bayesian statistical models, evaluating coverage, and annotating your consensus sequence. To learn more, see the Features or Resources tab below.

 

"SeqMan NGen is a very powerful application; a 454 run can be assembled to a reference genome in just a few minutes."

Elisabeth Navarro, CNRS

Features

Supported Workflows

  • Reference-guided assemblies for any size genome
  • De novo genome and transcriptome assemblies of up to 30 million sequences
  • Targeted re-sequencing/exome assemblies to identify low frequency SNPs and variants.
  • Metagenome and population assemblies and variant analysis
  • Genome Wide Association Studies (GWAS)

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    Fast and Accurate Assembler

  • Utilize our proprietary algorithms for fast and accurate assemblies on a desktop computer. See our benchmarking data for more information.
  • Take advantage of optimized assembly parameters for each of your projects, as well as the freedom to make adjustments if desired.Video See it!
  • Use paired-end sequence information during assembly, as well as for downstream analysis and contig ordering.

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    Post-Assembly Analysis

  • Open and navigate through large genomes for post-assembly analysis in SeqMan Pro with unprecedented ease and speed
  • Perform SNP and genotype calling in haploid and diploid organisms using Bayesian statistical models Video See it!
  • Do direct comparison to dbSNP during assembly for all major model organisms Video See it!
  • Analyze coverage depth, group contigs into scaffolds, and annotate your consensus Video See it!

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    Input/output Files

  • Import data from all major next-gen and third-gen platforms, including Illumina, Roche 454, SOLiD, and Ion Torrent.
  • Input multiplexed samples using standardized tag sets or user-defined custom tags.
  • Export as BAM, SAM, or ACE files.
  • Export your project to GenVision for a publication-quality graphical image of the assembly.

    • Resources

    Resources

    Whether you are a long time customer, or are just starting to learn about SeqMan NGen, you may find some of the following resources helpful:

    Technical Requirements

    Benchmarking Data

    Supplementary Files for Utilizing dbSNP Associations

    Licensing Options

    Assistance in Grant Preparation

     

    Training Videos:

    »See all SeqMan NGen Videos

    »Or browse our full Video Library

    Product Literature:

    Installation Guide - Windows and Macintosh

    Installation Guide - Linux

    SeqMan NGen Help Guide

    SeqMan NGen Scripting Manual

    Software Solutions for Next-Gen and Third-Gen Sequencing Technologies

    Next-Generation Sequencing...and Beyond Brochure

    Understanding Transcriptomes Solutions Using DNASTAR Software

    Human Genome Scale Assembly Poster

    Assembly and Annotation of Bacterial Genomes in Your Own Lab

     

    For additional resources, please see our Support page.

    Try It

    Try SeqMan NGen for Free!

    Request a fully functional free trial of SeqMan NGen to try it out for yourself.

     

    Free Trial for Windows

    Free Trial for Macintosh

    Free Trial for Linux - CentOS

    Free Trial for Linux - Ubuntu


    Buy It

    Ready to Buy?

    To purchase SeqMan NGen, please fill out our brief quote request form. Request a quote for SeqMan NGen only, or add other DNASTAR products at a multiple pricing discount.