Supported Sequencing Technology: Illumina

As an Illumina user, you will find that Lasergene Genomics Suite enables you to effortlessly assemble and analyze your sequencing data for the following project types:

De novo genome assemblies De novo transcriptome assemblies Reference-guided genome assemblies Targeted amplicon re-sequencing Whole genome/whole exome validation Large-scale, multi-sample SNP analysis ChIP-Seq and miRNA analysis Gene expression analysis

Our software uses parameters that have been optimized specifically for Illumina data and provides full support for Illumina multiplexed data, and paired-end sequence data.

 

Benchmarks: Illumina Reference-Guided Assembly

Data Set	Genome Size	Sequence Technology	Number of Reads	Number of Bases	Coverage	Assembly Time*	Gapped?
Rice Genome	272 Mbp	Illumina	272 M	8,900 M	32X	2 Hrs.	Y
Human Genome	3,095 Mbp	Illumina	3,521 M	117,000 M	38X	22 Hrs.	Y
Human Exome	3,095 Mbp	Illumina	155 M	4,200 M	50X	2 Hrs.	Y
RNA-Seq	3,095 Mbp	Illumina	128 M	3,500 M	NA	1.5 Hrs.	N

 

Benchmarks: Illumina De Novo Assembly

Data Set	Sequence Technology	Number of Reads	Number of Bases	Coverage	Contig N50	Assembly Time*
K-12 E. coli Genome	Illumina	1 M	115 M	25X	44 Kbp	38 Min.

*All projects completed on a desktop computer that Dell sells for < $3,000.

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