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Supported Sequencing Technology: Ion Torrent

Lasergene Genomics Suite seamlessly assembles and analyzes Ion Torrent sequencing data, making it the perfect complement for Ion Torrent users. Our software runs at unprecedented speed, even on low-cost desktop computers, enabling researchers to prepare a sample, generate sequence data, and assemble and analyze their data in a single day. DNASTAR Lasergene software supports the following Ion Torrent project types:

  • De novo genome assemblies
  • De novo transcriptome assemblies
  • Reference-guided genome assemblies
  • Targeted amplicon re-sequencing, including
    support for the Ion AmpliSeq™ Cancer Panel
  • Whole Genome/Whole Exome Validation
  • ChIP-Seq, RNA-Seq and miRNA analysis
    		•

    Our software uses parameters that have been optimized specifically for Ion Torrent data and provides full support Ion Torrent barcoded data, merge pair data, mate pair data, and paired-end sequence data and has recently significantly enhanced variant call accuracy in Ion Torrent data assemblies. 

     

    Request a fully-functional, free trial of Lasergene Genomics Suite.

     

     

    PDF Icon Using DNASTAR Software to Assemble and Analyze Ion Torrent Data: Genomes, Exomes, Targeted Resequencing and Beyond

     

    PDF Icon Ion Torrent Application Note Featuring SeqMan NGen

     

    "The software has been very simple to use. The DNASTAR team is working closely with Life Technologies to simplify the software for the everyday scientist to perform quick and efficient sequence analysis of genes of interest. I’m able to plug raw sequence data from the Ion Torrent server (i.e., FASTQ files) into the software suite and perform SNP analysis, multiple sequence alignments and phylogenetic trees. If I can do this type of bioinformatics, anyone can."

    Dr. Luke Daum, co-founder of Longhorn Vaccines & Diagnostics, Inc.

     

    Benchmarks: Ion Torrent Reference-Guided Assembly
    Data Set
    		 Sequence Technology
    Reference Sequence
    Genome Size (Mbp)
    Number of Reads (M)
    Number of Bases (Mbp)
    		 Read Length (bp) Coverage

    Assembly Time

    Ion AmpliSeq™ Cancer Panel (3 multiplexed data
    sets) Data provided by Ion Torrent.     		Ion Torrent
    3,101
    3
    99
    		 85 500X
    24 Min.
    K-12 E. coli Genome (merge pair data) Data provided by Ion Torrent.
    Merged pair data consists of overlapping forward and reverse reads.
    SeqMan NGen aligns these reads and merges them into a single consensus.
    Ion Torrent
    DH10B

    5

    3 M
    387
    115
    77X
    7 Min.
    RNA-Seq (2 human samples) Data provided by Ion Torrent.
    Ion Torrent
    3,101
    17.5 M
    875
    100
    NA
    1.5 Hrs.

     

    Benchmarks: Ion Torrent De Novo Assembly
    Data Set
    Sequence Technology
    Number of Reads (K)
    Number of Bases (M)
    Coverage

    Assembly Time
    K-12 E. coli Genome (merge pair data) Data provided by Ion Torrent.
    Merged pair data consists of overlapping forward and reverse reads.
    SeqMan NGen aligns these reads and merges them into a single consensus.
    Ion Torrent
    3,978
    387
    100X
    12
    2 Hrs.
    K-12 E. coli Genome (mate pair data) Data provided by Ion Torrent.
    Ion Torrent
    7,077
    1,826
    150X
    34
    3.5 Hrs.

    *All projects completed on a desktop computer that Dell sells for < $3,000.