DNASTAR - Lasergene SeqBuilder - Sequence editing, Annotation and Cloning

SeqBuilder - NEW Features Added in Lasergene v8.1

» Printable PDF overview for SeqBuilder

» View tutorial Training videos of SeqBuilder and other Lasergene applications

Sequence editing, mapping, annotation, and automated virtual cloning

SeqBuilder Image showing the development of a virtual clone

SeqBuilder enables users to edit nucleic acid and amino acid sequences and view sequences and related items in multiple ways. It also supports users to perform automated virtual cloning and primer design. SeqBuilder's window can be split into multiple panes so that more than one view can be displayed at any time. The possible views include a residue-specific view, a linear map, a circular map, a compact map of restriction cut sites (mini map), as well as a list of features, a summary of all restriction cut sites, and any comments.

Each view lists a number of items that can be individually shown including features, restriction sites, translations in all six reading frames, ORFs, and rulers. Functionality provides the ability to reverse complement, translate or back-translate sequences, identify ORFs and perform BLAST and Entrez text* searches directly to NCBI.

In general, the task of primer design in SeqBuilder is focused on creating a PCR insert from a region of interest. After a primer pair has been selected and modified as desired, SeqBuilder enables you to cut and clone the PCR insert into a vector using one of the cloning methods mentioned above.

SeqBuilder supports automated virtual cloning using several different cloning methods: restriction enzyme cloning; TA Cloning; Gateway Cloning; and Directional TOPO Cloning.

New Features found in Lasergene v8.1 include:

Multisite Gateway cloning capability
Updated Vector catalogues to include multisite cloning vectors
Improved export to GenVision for higher quality graphic images.

SeqBuilder Features

Create a New Cloning Project by adding (cut and paste) the insert you wish to clone
Select the method you wish to follow and the software will provide the appropriate vector selection from the Lasergene Vector Catalog
Add your own Vectors to the Vector Catalog
The Cloning Wizard will provide help if the ends to be cloned are not compatible
The wizard will construct the Virtual clone automatically after the ends and the vector selected are correct

Identify the region of interest, usually containing a gene.
Create primer pairs near the ends and in the same reading frame as the gene.
Select one pair from the search results in the Primer Design or Primer List view.
Modify the primers as desired.
Cut and clone the insert into a vector.

View sequences in a variety of forms including graphically rich circular and linear displays
Split the window to display multiple views of the same sequence simultaneously
Customize graphical and textual displays through simple curtain and click-and-drag interface
- Create, design and move graphic annotations
- Create default displays for specific types of annotations
- View double-stranded or single-stranded DNA, RNA or amino acid sequences
Create single page maps or multi-page posters
Interpret and display Genbank features graphically
Display lists of features, summary of cut sites and sequence comments
Display and save concise summaries of restriction sites sorted by classification
Copy and paste high-quality, resizable pictures into other software applications

Share sequence and feature edits simultaneously across multiple Lasergene applications
Open and save project files from GeneQuest, MegAlign, Protean and PrimerSelect

Create or edit constructs through standard editing and enhanced copy/paste functions
View ORFs that are updated with every sequence edit
Translate DNA or back translate protein using any of the supplied genetic codes or customize your own
Choose restriction sites to display based on any combination of:
- Manual selection of enzymes
- Frequency of cuts
- Type or class of enzyme
- Site complexity
- Overhang compatibility
Find related sequences using the integrated BLAST and Entrez* search tools
Editing the sequence automatically updates the feature coordinates

Import data from many popular file formats
Batch import Vector NTI files retaining feature names
Export data into the original file format automatically when the file format is one of several exportable formats offered
Download sequences directly from NCBI* using accession numbers, BLAST, or text searches

* Requires Internet Connection

» Printable PDF overview for SeqBuilder

» View a video tutorial of SeqBuilder

Multisite clone

Manage vectors, inserts, clones, and other sequences in project files

Automated virtual cloning for three protocols

Primer Design table in SeqBuilder

View the sequence, ORFs, translations

Enzyme manager view

Alter the display in many views, like this linear view