Sanger Sequence Assembly and Analysis

DNASTAR's Lasergene Core Suite software provides comprehensive support for assembly and analysis of Sanger sequencing data within the SeqMan Pro application. SeqMan Pro allows you to assemble your read data de novo or against one or more reference sequences. You can then use the analysis tools within the application to view read alignment and coverage, assess SNPs and annotate the consensus sequence. The software also allows you to:

Assemble your reads into groups based on sequence names

Trim vector and poor quality data and restore sequence ends

Automatically annotate variation features from the SNP Report and pull annotations from BLAST hits matching your consensus sequence

Design sequencing primers that walk into gaps or improve coverage

Export the consensus sequence to other applications for further analysis

Request a fully-functional, free trial of DNASTAR Lasergene.

For an overview of this workflow, please see the brief video below:

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