The Tree view shows evolutionary relationships predicted from the multiple sequence alignment.
- Click on the Tree tab at the bottom of the MegAlign Pro window. Press Neighbor Joining: BIONJ and then OK to calculate the tree.
The Tree view is calculated using the current Distance metric which is currently the default value Tamura-Nei (1993).
Note that the MegAlign Pro tree is always unrooted, regardless of the methods used for the multiple alignment and distance calculations.
- Hover your mouse over a node where branches meet and click on the minus sign symbol to collapse those branches. The branches collapse and are replaced by a triangle and text showing the number of branches that were collapsed.
- Select some of the uncollapsed branches by clicking on any vertical line towards the left of the tree. The selected branches are highlighted in blue.
- Now’s your chance to get creative. Change the look of the Tree view however you want using the Style panel’s Tree section. You can use the drop-down menus, change background and outline colors for various elements (by pressing the color box icons), and/or type different numbers into the text boxes. For a description of the many tree customization options, visit the topic Tree section.
- If desired, save the project using File > Save, or by clicking on the Save tool ().
This is the end of the Clustal Omega alignment tutorial.
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