The Stability – Isoelectric Precipitate track predicts regional instability by identifying uncharged regions at a given pH, which have an increased potential to aggregate if surface exposed. This method uses the pKa tables from Lehninger et al., 2005.
In the Tracks panel, expand Stability and check the box next to Stability – Isoelectric Precipitate. The track will now be visible in the Analysis view
Select the track in the Tracks panel. Open the Track Options section, which appears as follows:
- Residues to average – number of residues to be averaged when constructing each point in the plot. The default is 7.
- pH – Protean 3D highlights regions with a pI ± 0.5 pH units from the pH specified here. The default is 7.0.
Click if you wish to return to the default settings.
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