Assemble NGS data or large sets of Sanger data

When you elect to assemble multi-sample Sanger data from within SeqMan Ultra, you will be seamlessly transferred to SeqMan NGen to set up and run the assembly. Once assembly is complete, you can then press a button to launch the results in SeqMan Ultra for editing and analysis.

To assemble NGS data or large sets of Sanger data:

Do any of the following:

• Choose File > New Assembly with Options or press Ctrl/Cmd+Shift+N.

• Click on the Welcome project tab or choose View > Welcome to open the Welcome screen. Click on New Assembly on the left. On the right, choose New assembly with options or any of the Genomics or Transcriptomics options.

*Note: A padlock icon represents a workflow that is not available for your license type. A Lasergene Genomics license allows access to all workflows, while a Lasergene Molecular Biology license only allows you to access Sanger assembly workflows.

SeqMan NGen will open at the Workflow screen. Refer to the SeqMan NGen User Guide for detailed information about using the wizard. Help topics for some of the wizard screens you might encounter are listed below.

• Reference Sequence
• Input Sequences
• Assembly Options
• Analysis Options
• Assembly Output
• Run Assembly Project
• Assembly Summary.

Once assembly has finished, click the Open assembly button from the Assembly Summary screen to open the results in SeqMan Ultra for editing and analysis.