Step 1: Start a cloning project and specify the insert - User Guide to SeqBuilder Pro

The first step in performing a cloning simulation is to specify the insert. The following topics describe six different methods of specifying the insert to use with your cloning project:

Option A – Use a sequence file on your computer as the insert

Option B – Copy inserts from another SeqBuilder Pro cloning project

Option C – Use an entire open SeqBuilder Pro sequence as the insert

Option D – Use part of an open SeqBuilder Pro sequence file as the insert

Option E – Use the PCR product of the current primer pair as the insert

Option F – Use restriction enzyme cut sites to create the insert

Create a custom vector for directional TOPO cloning

Option A – Use a sequence file on your computer as the insert

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